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本研究采用亚克隆和序列修饰策略建立了编码人类乳头瘤病毒１６型（ＨＰＶ１６）主要结构蛋白Ｌ１基因的大肠杆菌可诱导表达质粒ｐＴｒｃ９９ＡｍＨＰＶ１６Ｌ１。通过限制性内切酶分析验证了ｐＴｒｃ９９ＡｍＨＰＶ１６Ｌ１的结构。初步表达显示携带ｐＴｒｃ９９ＡｍＨＰＶ１６Ｌ１的ＪＭ１０５在ＩＰＴＧ的诱导下可合成大小约５５ｋＤ的蛋白质，与预期结果一致
In this study, subclone and sequence modification strategy was used to construct E.coli inducible expression plasmid pTrc99Am-HPV16-L1 encoding the major structural protein L1 of human papillomavirus type 16 (HPV16). The structure of pTrc99Am-HPV16-L1 was verified by restriction enzyme analysis. Preliminary expression showed that JM105 carrying pTrc99Am-HPV16-L1 could synthesize about 55 kD protein under the induction of IPTG, which was consistent with the expected result