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将胚鼠中脑腹侧组织制成细胞悬液移植于帕金森病(PD)模型鼠侧脑室,动态观察受体鼠在移植前后的行为变化。移植后16周应用诱发荧光及免疫细胞化学等方法,对移植物在受体鼠脑内的存活及生长情况进行了研究。结果,受体鼠旋转行为明显改善,侧脑室内可见黄绿色荧光细胞及酪氨酸羟化酶(TH)免疫反应阳性细胞,有的发出突起伸向室管膜及迁于尾核区.提示:移植的胚鼠黑质细胞生长良好,似作为触液神经元发挥其作用。
The ventral midbrain tissue of the embryonic rat was made into cell suspension transplanted into the lateral ventricle of Parkinson’s disease (PD) model rats and the behavioral changes of the recipient mice were observed dynamically. At 16 weeks after transplantation, fluorescence and immunocytochemistry were used to study the survival and growth of the grafts in the recipient rat brain. As a result, the rotation behavior of the recipient mice was significantly improved, yellow-green fluorescent cells and tyrosine hydroxylase (TH) immunoreactive cells were observed in the lateral ventricle, and some protruded toward the ependymal membrane and moved to the caudateal nucleus. : Transplanted embryonic rat substantia nigra grows well and appears to play its role as a synaptic neuron.