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背景:迷走神经刺激参数直接关系到迷走神经刺激的抗痫效果及患者使用的安全性,选择适宜的迷走神经刺激参数组合是实现迷走神经刺激抗痫作用的关键因素。目的:观察电刺激参数包括输出电流、波宽、频率、开启时间、关闭时间、何时启动等在迷走神经刺激控制癫痫中的作用。设计:单一样本实验。单位:首都医科大学基础医学院神经生物学系。材料:实验于2000-09/2002-09在首都医科大学基础医学院神经生物学系电生理实验室完成。选择健康成年Wistar大鼠36只。方法:36只大鼠经海人藻酸复制大鼠癫痫模型,通过采用不同的迷走神经刺激参数组合,以大鼠行为学、心电图、皮层电图、海马神经元放电活动为观测指标,观察迷走神经刺激参数在控制致痫大鼠中的作用,并从中筛选适宜的刺激参数组合。主要观察指标:①海人藻酸致痫效应。②迷走神经刺激参数组合及其抗痫效应。结果:36只大鼠均进入结果分析。①迷走神经刺激的抗痫效应的观察主要在痫性发作4h以内。②当波宽为2ms,刺激强度为3～3.5mA,频率为30～35Hz,开启1min,关闭2min时,连续刺激迷走神经50min时,抗痫效果显著,痫性皮层电图的发作时间明显缩短,严重程度显著降低;致痫大鼠海马CA1区神经元暴发性放电频率降低(P<0.05)。结论:在保证受试者心电活动正常的前提下,能有效控制痫性发作的最小刺激参数组合为最适参数组合,且对心脏功能没有影响。
BACKGROUND: Parameters of vagal nerve stimulation are directly related to the anti-epileptic effect of vagus nerve stimulation and the safety of patients. Choosing the appropriate combination of vagal nerve stimulation parameters is the key factor to realize the anti-epileptic effect of vagus nerve stimulation. OBJECTIVE: To observe the role of electrical stimulation parameters in the control of epilepsy induced by vagus nerve stimulation, including output current, wave width, frequency, on time, off time and when to start. Design: Single sample experiment. SETTING: Department of Neurobiology, Capital Medical University. MATERIALS: Experiments were performed at the Department of Neurobiology, School of Basic Medicine, Capital Medical University between September 2000 and September 2002. Thirty-six healthy adult Wistar rats were selected. Methods: Totally 36 epilepsy rats were induced by alginic acid. Different vagal nerve stimulation parameters were used to observe the effects of vagus nerve stimulation on behavioral, electrocardiogram, electrocorticogram and hippocampal neuronal discharge. Parameters in the control of epileptic rats role, and to select the appropriate combination of stimulation parameters. MAIN OUTCOME MEASURES: ① kainic acid induced by epilepsy. ② vagus nerve stimulation parameters combination and its anti-epileptic effect. Results: All 36 rats were involved in the result analysis. ① Vagus nerve stimulation of the anti-epileptic effect mainly in epileptic seizures within 4h. ② When the wave width is 2ms, the stimulus intensity is 3 ~ 3.5mA, the frequency is 30 ~ 35Hz, on 1min, off 2min, the continuous stimulation of the vagus nerve 50min, the anti-epileptic effect is significant, epileptic electrocorticogram onset time was significantly shortened, The severity of neurons was significantly decreased. The frequency of neuronal outburst in hippocampal CA1 neurons decreased in epileptic rats (P <0.05). Conclusion: Under the premise of ensuring the normal electrocardiogram activity in subjects, the minimal stimulation parameter combination that can effectively control seizures is the optimal combination of parameters, and has no effect on cardiac function.