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【目的】了解肝硬化时大鼠肾上腺髓质素 (ADM )浓度是否改变 ;探讨外源性ADM(1 52 ) 对肝硬化大鼠门静脉压力的影响。【方法】以四氯化碳 (CCl4)诱导肝硬化大鼠模型 ,测定大鼠血浆ADM浓度。以ADM(1 52 ) 3nmol/kg ,经股静脉注射 ,持续监测大鼠门静脉压力的变化。测定肝硬化大鼠门静脉压力降至最低时内皮素 1(ET 1)及一氧化氮 (NO)的浓度。【结果】肝硬化大鼠血浆ADM浓度明显高于正常大鼠 (P <0 .0 1)。经股静脉注射ADM(1 52 ) 可迅速导致肝硬化大鼠门静脉压力显著下降 (P <0 .0 1) ,约 3.5min时下降至最低 ;约 9min基本恢复原水平。而正常大鼠注射ADM(1 52 ) 后 ,门静脉压力下降不明显 (P >0 .0 5 )。在肝硬化大鼠门静脉压力降至最低时 ,大鼠ET 1及NO浓度较未用药时无明显改变 (P >0 .0 5 )。【结论】血浆ADM浓度在肝硬化时升高 ;外源性ADM(1 52 ) 可显著降低肝硬化大鼠门静脉压力 ,而对正常大鼠门静脉压力无明显影响。ADM可能不是通过影响ET 1和NO的产生而发挥其降压作用。
【Objective】 To investigate whether adrenomedullin (ADM) concentration is changed in cirrhotic rats and to investigate the effect of exogenous ADM (125) on portal pressure in cirrhotic rats. 【Method】 CCl4-induced cirrhotic rat model was established and plasma ADM concentration was measured. ADM (125) 3nmol / kg, via the femoral vein injection, continuous monitoring of portal vein pressure changes. The levels of endothelin 1 (ET 1) and nitric oxide (NO) in the portal vein of cirrhotic rats were measured. 【Results】 The plasma ADM concentration in cirrhotic rats was significantly higher than that in normal rats (P <0.01). Intravenous injection of ADM (1 52) can rapidly lead to a significant decrease in portal pressure in cirrhotic rats (P <0.01), and then decrease to the lowest at about 3.5 min. However, no significant decrease in portal pressure was observed in normal rats after ADM (125) (P> 0.05). In the cirrhotic rat portal vein pressure is reduced to a minimum, the ET 1 and NO concentrations in rats had no significant change (P> 0.05). 【Conclusions】 The concentration of plasma ADM increased in cirrhotic rats. Exogenous ADM (1 52) significantly reduced the portal pressure of cirrhotic rats, but had no significant effect on portal pressure in normal rats. ADM may not exert its antihypertensive effect by affecting the production of ET 1 and NO.