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目的为成功构建恒河猴M1段栓塞模型提供简捷、迅速、安全的麻醉方法。方法雄性成年恒河猴25只,年龄7~9岁,体重7~11 kg,由中国人民解放军军事医学科学院实验动物中心提供。以0.1 ml/kg氯胺酮及速眠新的混合液肌肉注射,待动物入睡后气管插管(ID:4.5-5.5#),妥善固定后送入介入中心。入室后连接监护、建立静脉通路并导尿。术中用丙泊酚2~4 mg/(kg·h)持续泵入维持麻醉,呼吸机控制呼吸,并根据动物的生命体征和肢体活动情况调整麻醉深度,必要时追加上述氯胺酮与速眠新的混合液,并根据手术的需要调整心率、血压、体温等。溶栓前后行脑MRI造影,造影前适时停止麻醉药物输注,确保动物在造影室已恢复自主呼吸。术中监测实验动物的心率、体温、血压及血氧饱和度,并于股动脉穿刺及溶栓后抽取动脉血行血气分析。结果所有动物均按预计方案实施完成,术中未发生动物躁动、呼吸抑制、心律失常等严重并发症。实验结束停药后,实验动物很快清醒并送回动物实验中心,进行后续处理。其中18只实验动物存活24 h以上,7只死于溶栓后严重的脑出血和脑梗死。结论以氯胺酮及速眠新的混合液进行麻醉诱导行气管插管,丙泊酚静脉维持全身麻醉可以为此类较复杂的介入及MRI实验的顺利完成提供一种安全、实用的麻醉方法。
Objective To provide a simple, rapid and safe method of anesthesia for the successful construction of the rhesus monkey M1 segment embolization model. Methods Male adult rhesus monkeys 25, aged 7 to 9 years old and weighing 7 to 11 kg, were provided by the Experimental Animal Center of the Academy of Military Medical Sciences of the People’s Liberation Army. The rats were injected intramuscularly with 0.1 ml / kg ketamine and a mixture of Keshin-Miao. After the animals fell asleep, the animals were intubated (ID: 4.5-5.5 #) and properly fixed before they were delivered to the intervention center. Into the room after the connection monitoring, the establishment of venous access and catheterization. Intraoperative continuous infusion of propofol 2-4 mg / (kg · h) to maintain anesthesia, ventilator control of breathing, and according to the animal’s vital signs and physical activity to adjust the depth of anesthesia, if necessary, add the above ketamine and Supramoam Of the mixture, and according to the needs of surgery to adjust the heart rate, blood pressure, body temperature and so on. Thrombolysis before and after brain MRI, angiography stopped timely anesthesia infusion, to ensure that animals in the contrast chamber has resumed spontaneous breathing. Intraoperative monitoring of heart rate, body temperature, blood pressure and oxygen saturation in experimental animals, and femoral artery puncture and thrombolysis after arterial blood gas analysis. Results All the animals were completed according to the plan. There were no serious complications such as agitation, respiratory depression and arrhythmia during operation. After stopping the experiment, the experimental animals were quickly awake and sent back to the animal experiment center for follow-up treatment. Among them, 18 animals survived more than 24 hours, and 7 died of severe cerebral hemorrhage and cerebral infarction after thrombolysis. Conclusion The anesthesia induced by ketamine and Hsiehmian new mixed liquid can be used for tracheal intubation and propofol intravenous anesthesia can provide a safe and practical method for anesthesia in this complex intervention and MRI experiment.