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目的建立测定金果榄药材中古伦宾和蝙蝠葛任碱含量的胶束电动毛细管色谱法。方法采用未涂层熔融石英毛细管柱(35.2 cm×50μm I.D.,有效长度为26.9 cm),运行缓冲液为20 mmol.L-1硼酸溶液(含200 mmol.L-1十二烷基硫酸钠,pH值8.0)-异丙醇-甲醇(体积比70∶20∶10),分离电压为18 kV,检测波长为210 nm,压力自动进样。结果古伦宾和蝙蝠葛任碱的线性范围分别为0.210～1.68 g.L-1和0.026～0.21 g.L-1,线性关系良好。平均加样回收率分别为100.5%和96.1%,RSD分别为3.4%和3.6%(n=6)。应用该法测定10批金果榄药材中古伦宾和蝙蝠葛任碱的含量。结论该方法准确、可靠、环保,适用于金果榄中古伦宾和蝙蝠葛任碱的同时含量测定。
OBJECTIVE To establish a micellar electrokinetic capillary chromatography method for the determination of allrenaline in batraceae and bats. Methods Uncoated fused silica capillary column (35.2 cm × 50 μm ID, effective length of 26.9 cm) was used. The running buffer consisted of 20 mmol·L-1 boric acid solution containing 200 mmol·L-1 sodium lauryl sulfate, pH 8.0) - isopropanol - methanol (70:20:10 by volume). The separation voltage was 18 kV and the detection wavelength was 210 nm. The pressure was injected automatically. Results The linear range of gullumbin and bat kern was 0.210-1.68 g · L-1 and 0.026-0.21 g · L-1, respectively. The linearity was good. The average recovery rates were 100.5% and 96.1%, respectively, with RSDs of 3.4% and 3.6%, respectively (n = 6). The method was used to determine the content of glebone and batsinequine in 10 batches of Jinguolu medicinal materials. Conclusion The method is accurate, reliable and environmentally friendly and suitable for the simultaneous determination of glebolins and bats allogene in Camellia.