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提出间氨基酚 ( MAP) -H2 O2 -辣根过氧化物酶 ( HRP)伏安酶联免疫分析新体系 ,并用于南方菜豆花叶病毒 ( SBMV)的测定 .以线性扫描二阶导数伏安法检测 HRP催化 H2 O2 氧化 MAP的产物 ,用于游离 HRP及 SBMV的测定 ,灵敏度均高于经典的 ELISA显色光度法 .本法对 HRP测定的线性范围为 1 .0× 1 0 - 8～1 .0× 1 0 - 6 g/L,检测限为 3 .8× 1 0 - 9g/L;对 SBMV测定的线性范围为 4 .0～ 50 0 0 ng/m L,检测限为 4 .0ng/m L.用所建立的方法测定病毒感染病叶澄清液的最高稀释比为 1∶ 1 .5× 1 0 5 ,并与现行的 ELISA显色光度法进行对照 ,二者相关性很好
A new system of enzyme - linked immunosorbent assay (HRP) was developed for the determination of SBMV by MAP - H2O2 - horseradish peroxidase (HRP) enzyme linked immunosorbent assay (ELISA) The results showed that HRP catalyzed the oxidation of MAP product by H2O2 for the determination of free HRP and SBMV and the sensitivity was higher than that of the classical ELISA colorimetric method.The linear range of HRP determination was 1.0 × 10 ~ The detection limit was 3. 8 × 10 ~ 9 g / L. The linear range of SBMV was 4.0-0.00 0 ng / m L with a detection limit of 4. 0ng / m L. The maximum dilution ratio of the virus-infected leaf serum was 1: 1.5 × 105 determined by the established method and compared with the current ELISA colorimetric method, the correlation between them was very good