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目的研究血管内皮高表达CYP2C8基因能否改善小鼠动脉粥样硬化。方法以20和40周龄APOEKO+/-CYP2C8Tg+/-和CYP2C8Tg+/-基因(血管内皮特异性CYP2C8+/-转基因)的小鼠(n=10/组)为研究对象,相同基因背景和周龄的同窝APOEKO+/-和C57BL/6小鼠设为对照。采用PCR技术鉴定小鼠CYP2C8+/-基因;油红O染色检测APOEKO+/-CYP2C8Tg+/-、CYP2C8Tg+/-、APOEKO+/-和C57BL/6小鼠主动脉斑块形成面积;酶比色检测APOEKO+/-CYP2C8Tg+/-、CYP2C8Tg+/-、APOEKO+/-和C57BL/6小鼠血清TG、TCH、HDL。结果 20和40周龄APOEKO+/-CYP2C8Tg+/-和CYP2C8Tg+/-小鼠主动脉斑块形成面积与同周龄野生型小鼠相比明显减少,并且明显改善了血脂代谢状态。结论高表达CYP2C8基因能够改善西方饮食诱导的不同周龄小鼠的动脉粥样硬化。
Objective To study whether high expression of vascular endothelium CYP2C8 gene can improve atherosclerosis in mice. Methods Mice (n = 10 / group) with APOEKO +/- CYP2C8Tg +/- and CYP2C8Tg +/- genes (vascular endothelium-specific CYP2C8 +/- transgene) at 20 and 40 weeks of age were studied. The same genetic background and age Nest APOEKO +/- and C57BL / 6 mice were set as controls. The CYP2C8 +/- gene was identified by PCR. The area of aortic plaque formation in APOEKO +/- CYP2C8Tg +/-, CYP2C8Tg +/-, APOEKO +/- and C57BL / 6 mice was detected by oil red O staining. The enzymatic colorimetric assay APOEKO +/- Serum TG, TCH, HDL of CYP2C8Tg +/-, CYP2C8Tg +/-, APOEKO +/- and C57BL / 6 mice. Results APOEKO +/- CYP2C8Tg +/- and CYP2C8Tg +/- mouse plaque area formation at 20 and 40 weeks of age was significantly reduced compared to wild-type mice of the same age and significantly improved lipid metabolism status. Conclusion High expression of CYP2C8 gene can improve the atherosclerosis induced by western diet in mice of different weeks.