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采用基于注射挤压器的液滴形成技术制备包裹了苏云金杆菌晶体和芽胞的海藻酸钙凝胶微球。通过调节该装置的活塞重量和空气压力,获得了平均直径为20μm的微球。SDS-PAGE分析与平板菌落涂布实验表明,凝胶微球可有效减少紫外线对苏云金杆菌晶体和芽胞的损伤作用。利用小菜蛾进行的毒力生测发现,凝胶微球可有效防止紫外线引起的晶体和芽胞杀虫毒力的下降。本研究的液滴形成技术也可适用于其它微球包裹过程。
Calcium alginate gel microspheres wrapped with Bacillus thuringiensis crystals and spores were prepared using an injection-based drop formation technique. By adjusting the piston weight and the air pressure of the device, microspheres with an average diameter of 20 μm were obtained. SDS-PAGE analysis and plate colony coating experiments show that the gel microspheres can effectively reduce the UVB Bacillus thuringiensis crystal and spores of the injury. Virulence test using Plutella xylostella revealed that the gel microspheres can effectively prevent the UV-induced drop in the insecticidal toxicity of crystal and spore. The droplet formation technique of this study can also be applied to other microsphere encapsulation processes.