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为了分析MNC对二硝基氟苯(DNFB)诱导的小鼠迟发型超敏反应(DTH)的药理效应,探讨其在体内免疫抑制作用的机制,我们采用0.5%DNFB腹部致敏,耳廓激发的方法建立小鼠DTH模型。激发后48 h,取小鼠双耳称重分析MNC对DTH模型小鼠耳组织肿胀、炎性细胞浸润度和胸腺指数、脾脏指数的影响;光学显微镜下观察小鼠耳廓组织学变化;噻唑兰(MTT)法检测淋巴结淋巴细胞和脾脏淋巴细胞增殖情况。双耳称重结果显示,MNC(70 mg/ml)处理组与DTH组相比,能明显抑制DNFB引起的炎症反应(P<0.05);耳廓局部组织学检测也表明,MNC能够抑制DNFB诱导的DTH,明显减少淋巴细胞的浸润;MNC能降低DTH小鼠胸腺指数和脾脏指数,抑制淋巴结淋巴细胞(P<0.05)和脾脏淋巴细胞的增殖(P<0.05)。实验结果提示,MNC可显著抑制DTH小鼠耳组织肿胀和淋巴细胞浸润程度,降低胸腺和脾脏指数,抑制淋巴细胞的增殖可能是其作用机制之一。
In order to analyze the pharmacological effects of MNC on dinitrofluorobenzene (DNFB) -induced delayed-type hypersensitivity (DTH) in mice and its mechanism of in vivo immunosuppression, we used 0.5% DNFB for abdominal sensitization, auricle excitement The mouse DTH model was established. Forty-eight hours after challenge, mice were weighed and analyzed by binocular MNC. The effects of MNC on ear tissue swelling, inflammatory cell infiltration, thymus index and spleen index in mice with DTH were observed. The auricle histological changes were observed under light microscope. Blue (MTT) assay lymph node lymphocyte and spleen lymphocyte proliferation. Binaural weighing showed that MNC (70 mg / ml) treatment significantly inhibited DNFB-induced inflammatory response compared with DTH group (P <0.05). Local auricular histological examination also showed that MNC inhibited DNFB induction DTH significantly reduced lymphocyte infiltration. MNC decreased the thymus index and spleen index of DTH mice, and inhibited lymph node lymphocyte (P <0.05) and spleen lymphocyte proliferation (P <0.05). The experimental results suggest that MNC can significantly inhibit the ear tissue swelling and lymphocyte infiltration of DTH mice, reduce thymus and spleen index, and inhibit the proliferation of lymphocytes may be one of the mechanisms.