论文部分内容阅读
目的:观察胃癌组织中RUNX3基因启动子区甲基化状态及其对蛋白表达的影响,并分析甲基化及蛋白表达与临床病理特征的关系。方法:采用甲基化特异性定量PCR检测胃癌组织及相应癌旁正常组织中RUNX3的甲基化状态。应用Western Blot法检测RUNX3蛋白表达情况。结果:162例胃癌组织及相应癌旁组织中RUNX3基因甲基化阳性率分别为77.8%(126/162)、38.9%(63/162)(P<0.001)。RUNX3蛋白表达下调/缺失率分别为81.5%(132/162)、12.3%(21/162)(P<0.001)。RUNX3基因甲基化阳性率与胃癌的临床分期、组织分化程度、肿瘤直径、淋巴结转移具有相关性(P<0.05),RUNX3蛋白表达与胃癌的组织分化程度、淋巴结转移具有相关性(P<0.05)。结论:RUNX3甲基化及其蛋白表达与胃癌的发生发展关系密切,并且甲基化影响其蛋白表达。
OBJECTIVE: To observe the methylation status of RUNX3 promoter and its effect on protein expression in gastric cancer, and to analyze the relationship between methylation and protein expression and clinicopathological features. Methods: Methylation-specific quantitative PCR was used to detect the methylation status of RUNX3 in gastric cancer tissues and adjacent normal tissues. Western Blot method was used to detect RUNX3 protein expression. Results: The positive rates of RUNX3 methylation in 162 cases of gastric cancer tissues and adjacent tissues were 77.8% (126/162) and 38.9% (63/162), respectively (P <0.001). The down-regulation and down-regulation of RUNX3 protein expression were 81.5% (132/162) and 12.3% (21/162), respectively (P <0.001). The positive rate of RUNX3 methylation was correlated with clinical stage, histological differentiation, tumor diameter and lymph node metastasis (P <0.05). The expression of RUNX3 was correlated with the degree of differentiation and lymph node metastasis (P <0.05) ). Conclusion: RUNX3 methylation and its protein expression are closely related to the occurrence and development of gastric cancer, and methylation affects its protein expression.