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目的研究葛根素对性激素水平及前列腺增生组织中雌激素α受体的影响。方法取50只昆明小鼠,乙醚浅麻醉,常规消毒,摘除双侧睾丸,缝合创口。术后第3天,各组每天皮下注射丙酸睾酮5.0 mg·kg-1·d-1,连续12 d,制造小鼠前列腺增生模型。50只小鼠随机分为5组,正常对照组,前列腺增生模型组,5.0,10.0,20.0 mg·kg-1·d-1葛根素组,观察各种处理12 d后小鼠前列腺湿重、前列腺指数、性激素水平及前列腺组织中雌激素α受体表达的影响。结果与模型组比较,10.0,20.0 mg·kg-1·d-1葛根素能显著抑制丙酸睾丸酮所致小鼠前列腺增生;明显降低小鼠血清睾丸酮(T)和雌二醇(E2)及增生组织中雌激素受体的表达。结论葛根素对丙酸睾丸酮所致小鼠前列腺增生具有显著的拮抗作用;其作用机制可能一定程度上与降低小鼠血清T,E2,T/E2及抑制雌激素α受体的表达有关。
Objective To study the effects of puerarin on the levels of sex hormones and estrogen α receptors in benign prostatic hyperplasia. Methods Fifty Kunming mice were killed, anesthetized by light anesthesia, routinely sterilized, bilateral testis removed, and sutured. On the third day after operation, testosterone propionate 5.0 mg · kg-1 · d-1 was injected subcutaneously into the rats in each group for 12 days continuously to establish a model of prostatic hyperplasia in mice. 50 mice were randomly divided into 5 groups: normal control group, benign prostatic hyperplasia model group, 5.0, 10.0, 20.0 mg.kg-1d-1 puerarin group, Prostate Index, Sex Hormone Levels and Estrogen Receptor Expression in Prostate Tissue. Results Compared with model group, puerarin 10.0, 10.0 mg · kg-1 · d-1 could significantly inhibit the testosterone-induced prostatic hyperplasia in mice and the serum testosterone (T) and estradiol (E2) Expression of estrogen receptor in hyperplastic tissues. CONCLUSION Puerarin has a significant antagonistic effect on testosterone-induced prostatic hyperplasia in mice. The mechanism may be related to the decrease of serum T, E2, T / E2 and the inhibition of estrogen-α receptor expression in mice.