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前言在体外应用组织培养的方法,使肿瘤细胞以多细胞聚集体的形式生长,这种具有三维结构的细胞团,称为多细胞肿瘤体(MTS)。由于MTS 在组织结构、生长动力学、对辐射、药物和宿主免疫反应等方面与实体瘤的性质相同,故MTS 被称为肿瘤的体外模型。MTS 的培养法及其影响因素一、MTS 的培养法大致可分为:(一)搅拌瓶培养法:通常孵育二周后球体就可使用。(二)静止培养法:将细胞悬液接种到
Introduction In vitro tissue culture methods allow tumor cells to grow in the form of multicellular aggregates. This three-dimensional structure of cell clusters is called multicellular tumor (MTS). Because MTS has the same properties as solid tumors in terms of histological structure, growth kinetics, and radiation, drug, and host immune response, MTS is called an in vitro model of tumors. MTS culture method and its influencing factors 1. The culture method of MTS can be roughly divided into: (1) Shaking flask culture method: Generally, the sphere can be used after two weeks of incubation. (B) Static culture method: inoculate cell suspension into