论文部分内容阅读
本实验在体外测试了两种新的杀丝虫药物CGP6140和CGP20376对盘尾丝虫微丝蚴的作用。药物在试验前溶解于丙酮溶液中(丙酮:蒸馏水为1:1),再稀释至试验所需的浓度。微丝蚴取自利比里亚盘尾丝虫病患者切除的结节内,按Ham等法(1981),将损伤边缘的皮肤片切下后低温保存于60％培养基199、20％胎牛血清和20％甲醇的溶液,置于液氮中。试验时在35℃下速溶,然后在新鲜培养基中孵育,使微丝蚴游离。微丝蚴经150g离心10分钟,用无血清培养基洗3次,于试验板24井中每井置1ml培养基和80～100条微丝蚴。培养基的组成是:IMDM/NCTC135的1:1混合物、Hepes、20％热灭
In this study, we tested the effects of two novel nematocidal drugs CGP6140 and CGP20376 on Microfilaria of onchocerciasis in vitro. The drug was dissolved in an acetone solution (1: 1 acetone: distilled water) prior to testing and then diluted to the concentration required for testing. Microfilaria was collected from nodules excised from Liberian filariasis in Liberian. According to the Ham et al. (1981), the lesioned skin slices were excised and stored in 60% medium 199, 20% fetal bovine serum and 20% methanol solution, placed in liquid nitrogen. At 35 ° C, the solution was quickly dissolved and then incubated in fresh medium to release microfilariae. Microfilaria was centrifuged at 150 g for 10 minutes and washed three times with serum-free medium. One ml of culture medium and 80 to 100 microfilariae were placed in well 24 in the test plate. The medium consisted of: a 1: 1 mixture of IMDM / NCTC135, Hepes, 20% heat-off