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将稀土纳米材料Er_2O_3用于构建葡萄糖生物传感器。Er_2O_3和氧化石墨烯形成复合基底,将葡萄糖氧化酶(GOD)固载在玻碳电极表面。首先利用SEM和XRD技术对所制备的Er_2O_3和氧化石墨烯纳米材料进行表征。利用EIS和CV对整个生物传感器制备过程进行表征。Er_2O_3的存在能有效地保持GOD的生物活性并加速其与电极之间的电子传递。由于Er_2O_3和氧化石墨烯之间的协同效应,使得制备的传感器在CV图中呈现一对明显的氧化还原峰,证实GOD和电极之间的直接电子传递性能。当用于对葡萄糖的电催化氧化时,传感器的CV响应随着葡萄糖浓度的增加而变弱。在葡萄糖浓度为1~10 mmol·L-1范围内,CV响应值与葡萄糖浓度成线性关系。此外,传感器具有好的稳定性和重现性。
The rare earth nanometer material Er_2O_3 was used to construct glucose biosensor. Er 2 O 3 and graphene oxide to form a composite substrate, the glucose oxidase (GOD) immobilized on the glassy carbon electrode surface. First, the prepared Er 2 O 3 and graphene oxide nanomaterials were characterized by SEM and XRD techniques. The entire biosensor preparation process was characterized using EIS and CV. The existence of Er_2O_3 can effectively maintain the biological activity of GOD and accelerate the electron transfer between the electrode. Due to the synergistic effect between Er 2 O 3 and graphene oxide, the prepared sensor showed a pair of obvious redox peaks in the CV image, confirming the direct electron transfer between GOD and the electrode. When used for the electrocatalytic oxidation of glucose, the sensor’s CV response becomes weaker with increasing glucose concentration. In the glucose concentration of 1 ~ 10 mmol · L-1 range, CV response linearly with glucose concentration. In addition, the sensor has good stability and reproducibility.