【摘 要】
：
Background:Invasive Trichosporon asahii (T.asahii) infection frequently occurs with a high mortality in immunodeficient hosts,but the pathogenesis of T.asahii infection remains elusive.Circular RNAs (circRNAs) are a type of endogenous noncoding RNA that p
【机 构】
：
Department of Dermatology,Southwest Hospital,Army Medical University,Chongqing 400038,China;Departme
论文部分内容阅读
Background:Invasive Trichosporon asahii (T.asahii) infection frequently occurs with a high mortality in immunodeficient hosts,but the pathogenesis of T.asahii infection remains elusive.Circular RNAs (circRNAs) are a type of endogenous noncoding RNA that participate in various disease processes.However,the mechanism of circRNAs in T.asahii infection remains completely unknown.Methods:RNA sequencing (RNA-seq) was performed to analyze the expression profiles of circRNAs,microRNAs(miRNAs),and mRNAs in THP-1 cells infected with T.asahii or uninfected samples.Some of the RNA-seq results were verified by RT-qPCR.Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG)pathway analyses were used to analyze the differentially expressed mRNAs.A circRNA-miRNA-mRNA network was constructed and verified by dual-luciferase reporter assay and overexpression experiments.Results:A total of 46 circRNAs,412 mRNAs and 47 miRNAs were differentially expressed at 12 h after T.asahii infection.GO and KEGG analyses showed that the differentially expressed mRNAs were primarily linked to the leukocyte migration involved in the inflammatory response,the Toll-like receptor signaling pathway,and the TNF signaling pathway.A competing endogenous RNA (ceRNA) network was constructed with 5 differentially expressed circRNAs,5 differentially expressed miRNAs and 42 differentially expressed mRNAs.Among them,hsa circ 0065336 was found to indirectly regulate PTPN11 expression by sponging miR-505-3p.Conclusions:These data revealed a comprehensive circRNA-associated ceRNA network during T.asahii infection,thus providing new insights into the pathogenesis of the T.asahii-host interactions.
其他文献
人体解剖学是高职高专院校护理专业的专业基础课 [1].资料显示,100％的护理技能操作涉及到解剖学知识.全国护士执业资格考试(以下简称“护考”)大纲列出了各章节疾病护理必需掌握的解剖学结构概要 [2].因此,熟练掌握解剖学知识对于通过“护考”具有重要的意义.有些学者也对以“护考”为导向的人体解剖学教学进行了探索.闫郭莉 [3]对如何高效计划和组织解剖学课程和如何对教育过程的计划与组织进行判断提出了看法,并设计了一个评价项目和标准.蔡宏宇?[4]对护士资格考试大纲导入解剖学的必要性进行了分析,并提出了基于护
笔者进行标本制作时,发现腰静脉异常的成年男性一例,右侧仅2支腰静脉经下腔静脉后壁汇入下腔静脉,左侧见3支腰静脉全部汇入左肾静脉,未见左侧腰升静脉存在.经作者查询国内相关文献,此种变异属较罕见.
晶状体病是眼科教学中比较重要的一个章节 [1],由于此章节中涉及的眼科教学重点疾病-白内障也是临床防盲治盲工作中的主要疾病,所以教学效果上的要求不同于眼科学中的其他章节,此章节的教学效果要求做到学生必须弄懂且确保掌握 [2].如何保证教学效果?本研究根据以往的教学经验,创造出以学生比较熟悉的解剖结构为先导,顺其自然地导入需要学生掌握的重点教学内容,用这种教学方法取得了比较好的效果.
Trauma-induced coagulopathy (TIC) is caused by post-traumatic tissue injury and manifests as hypercoagulability that leads to thromboembolism or hypocoagulability that leads to uncontrollable massive hemorrhage.Previous studies on TIC have mainly focused
目的 研究经支气管镜活瓣(EBV)植入肺减容术治疗重度肺气肿的疗效.方法 回顾性分析从2016年10月～2019年2月,于我院实施EBV植入肺减容术治疗的36例重度肺气肿患者的相关病历资料,记为研究组.另取从2013年2月～2015年6月,于我院接受开胸肺减容术治疗的36例重度肺气肿患者作为对照组.比较治疗前后两组肺功能、生活质量、动脉血气分析以及并发症发生情况.结果 治疗后12个月观察组的各项肺功能指标水平和对照组相比均不明显(P>0.05).观察组治疗后12个月PaCO2、PaO2水平和对照组相比均不
Evidence shows that pulmonary problems in coronavirus disease 2019 (COVID-19) may set off from vascular injury that progresses to physiological disturbances through a compromised gas exchange,following an infection with the severe acute respiratory syndro
Wound healing,tissue repair and regenerative medicine are in great demand,and great achievements in these fields have been made.The traditional strategy of tissue repair and regeneration has focused on the level of tissues and organs directly;however,the
目的 本研究旨在探讨血清学标志物在原发性干燥综合征继发间质性肺病(pSS-ILD)患者诊断和病情严重程度评估中的作用.方法 回顾性分析2013年5月至2014年5月南京鼓楼医院31例间质性肺病继发干燥综合征患者的临床资料,并进行电话随访,肺炎及健康体检者作为对照组,检测患者血清涎液化糖链抗6(KL-6)、白细胞介素-4(IL-4)、肺表面活性蛋白-D(SP-D)和转化生长因子-β(TGF-β)表达水平,分析其与疾病严重程度的关系.结果 pSS-ILD患者血清KL-6水平显著高于肺炎组及健康对照组,KL-6
目的 研究老年慢性阻塞性肺疾病(COPD)合并肺部真菌感染患者痰液标本病原菌分布情况及外周血presepsin、Hepcidin-25水平变化意义.方法 选取本院于2018年3月-2020年3月收治的102例老年COPD合并肺部真菌感染患者为研究对象,定义为合并感染组,并对其随访1年.同时选取同时期在本院就诊的单纯COPD患者88例,定义为COPD组,并招募62例年龄、性别与上述两组相匹配的健康志愿者,定义为对照组.观察合并感染组患者痰液标本病原菌分布情况.检测三组外周血presepsin、Hepcidi
目的 探讨不同内型哮喘小鼠模型中,小气道功能是否存在异常及其相关机制.方法 卵清蛋白(OVA)致敏、激发建立T2型哮喘模型,OVA联合臭氧暴露(OVA+ozone)建立非T2型哮喘模型.模拟强迫振荡系统检测小鼠小气道功能,激发试验检测气道反应性.酶联免疫吸附试验法检测支气管肺泡灌洗液(BALF)中的细胞因子;苏木精-伊红染色法对肺切片进行病理分析,免疫组织化学方法检测肺组织中的α-平滑肌肌动蛋白.结果 T2型和非T2型哮喘模型中均存在大、小气道功能障碍,显著的气道炎症、气道高反应性以及气道重塑,并有BAL