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目的探讨用超声雾化石棉悬液建立大鼠石棉肺模型的可行性。方法选取体重在180~200 g的雄性健康SD大鼠40只,随机分为正常对照组5只和实验组35只(实验组分为7个亚组,分别为1、3天和1、2、4、8、10周亚组,每亚组5只)。将石棉纤维制成石棉纤维悬液,超声雾化让动物吸入,每天雾化吸入2 h,每周吸入7 d,最长染尘10周。以肺组织中丙二醛(MDA)、超氧化物歧化酶(SOD)、组织羟脯氨酸(Hyp)、肺脏指数、肺组织病理改变为观察指标。结果实验组肺脏指数实验初2周内较正常对照组高,而2周以后体重变化增长较正常对照组慢;MDA与SOD变化规律相似,均为先增高后减低,MDA 2周达峰值,而SOD 1周达峰值;Hyp于1周以后逐渐升高。肺组织早期以充血为主,中后期则以慢性炎症为主,随时间延长,纤维化逐渐加重,纤维化呈弥散性,但以血管周围及细支气管周围最为严重。结论采用超声雾化染尘方法可以方便地建立大鼠石棉肺模型。
Objective To investigate the feasibility of using asbestos suspension to establish asbestos lung model in rats. Methods Forty healthy male Sprague-Dawley rats weighing 180 ~ 200 g were randomly divided into five groups (n = 5) and experimental group (n = 7) , 4, 8, 10 weeks subgroup, 5 subgroups). The asbestos fiber suspension made of asbestos fibers, ultrasonic atomization of the animals inhaled, daily inhalation inhalation 2 h, 7 d inhalation weekly, the longest dust for 10 weeks. Malondialdehyde (MDA), superoxide dismutase (SOD), tissue hydroxyproline (Hyp), lung index and pathological changes in lung tissue were observed as indicators. Results The lung index of the experimental group was higher than that of the normal control group in the first 2 weeks, but slower than that of the normal control group after 2 weeks. The changes of MDA and SOD were similar, both increased and then decreased, while MDA reached the peak value in 2 weeks SOD peaked at 1 week and Hyp gradually increased after 1 week. Early congestion of lung tissue mainly in the late chronic inflammation is the main, with time, gradually increased fibrosis, fibrosis was diffuse, but to the perivascular and bronchioles around the most serious. Conclusion Asbestos lung model can be easily established by ultrasonic atomizing and dusting method.