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目的:检测钙敏感受体(CaSR)在高肺血流性肺动脉高压(PH)大鼠肺动脉平滑肌中的表达水平及血浆中H2S含量,阐明两者在PH形成机制中的作用。方法:18只雄性SD大鼠随机分为对照组和PH组,对照组大鼠行单纯开胸,PH组大鼠行左肺切除手术。饲养35d后,应用右心导管法分别测定各组大鼠肺动脉平均压(PAMP),检测右心室/体质量(RV/BW)和右心室/左心室+室间隔[RV/(LV+S)]比值,光学显微镜观察肺血管结构的变化,分光光度法检测血浆H2S含量,应用实时荧光定量PCR方法检测大鼠肺动脉平滑肌细胞CaSR mRNA的表达水平。结果:PH组大鼠PAMP、RV/BW及RV/(LV+S)比值均明显高于对照组(P<0.05),光镜下肺小血管肌化程度明显增强,肺中、小肌型动脉相对中膜厚度明显增加(P<0.05)。PH组大鼠肺动脉平滑肌细胞CaSR mRNA的表达水平较对照组明显增高(P<0.05);PH组大鼠血浆中H2S含量较对照组明显降低(P<0.05)。大鼠肺动平滑肌细胞CaSR mRNA的表达水平与血浆H2S含量呈负相关(r=-0.931,P<0.05)。结论:高肺血流时肺动脉平滑肌细胞CaSR mRNA表达的改变可能通过改变内源性H2S的产生参与PH的形成。
AIM: To detect the expression of calcium-sensitive receptor (CaSR) in pulmonary artery smooth muscle of rats with pulmonary hypertension of pulmonary hypertension (PH) and the content of H2S in plasma and to elucidate their roles in the mechanism of PH formation. Methods: Eighteen male Sprague-Dawley rats were randomly divided into control group and PH group. Rats in control group were treated by simple thoracotomy and PH group rats underwent left lung resection. After feeding for 35 days, the mean pulmonary arterial pressure (PAMP) and right ventricular mass (RV / BW) and right ventricular / left ventricular + interventricular septum [RV / (LV + S) ] Ratio, the changes of pulmonary vascular structure were observed with light microscope, the content of H2S in plasma was detected by spectrophotometry, and the expression of CaSR mRNA in pulmonary artery smooth muscle cells was detected by real-time fluorescence quantitative PCR. Results: The ratios of PAMP, RV / BW and RV / (LV + S) in PH group were significantly higher than those in control group (P <0.05) Arterial relative media thickness was significantly increased (P <0.05). Compared with the control group, the expression of CaSR mRNA in pulmonary arterial smooth muscle cells of PH group was significantly increased (P <0.05); the content of H2S in plasma of PH group was significantly lower than that of the control group (P <0.05). The level of CaSR mRNA in rat pulmonary artery smooth muscle cells was negatively correlated with plasma H2S level (r = -0.931, P <0.05). Conclusion: The changes of CaSR mRNA expression in pulmonary artery smooth muscle cells may be involved in the formation of PH by altering the production of endogenous H2S.