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目的研究亚硒酸钠与核黄素联合暴露对高脂饮食雄性大鼠血脂及血清肝生化指标的影响。方法将60只健康SPF级雄性SD大鼠按体重随机分为空白对照组(10只)和高脂组(50只),分别给予基础饲料和高脂饲料;喂养4周后,将50只高脂组大鼠按照血脂水平和体重随机分为5组,分别为高脂对照组、1.84μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组、18.40μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组、1.84μg/kg亚硒酸钠+3.50 mg/kg核黄素干预组和18.40μg/kg亚硒酸钠+3.50 mg/kg核黄素干预组,每组10只。采用灌胃方式进行染毒,同时,空白对照组和高脂对照组给予相同体积的生理盐水,染毒容量为10 ml/kg,每天1次,连续60 d。分别于第0(染毒前)、20、40、60天,测定大鼠血清中总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)和高密度脂蛋白胆固醇(HDL-C)水平;并于第60天,检测大鼠血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转氨酶(AST)的水平。结果与空白对照组比较,染毒期间高脂对照组及各干预组大鼠血清TC、TG、LDL-C的水平均升高,除第60天时1.84μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组大鼠血清TG水平外,差异有统计学意义(P<0.05);而血清HDL-C水平均未见显著变化。与高脂对照组相比,第20、40天时1.84μg/kg亚硒酸钠+0.70mg/kg核黄素干预组大鼠血清TC、TG的水平及1.84μg/kg亚硒酸钠+3.50 mg/kg核黄素干预组大鼠血清TG的水平以及第60天时1.84μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组大鼠血清TC、TG、LDL-C的水平及18.40μg/kg亚硒酸钠+0.70mg/kg核黄素干预组大鼠血清TC的水平均下降,差异有统计学意义(P<0.05);而各干预组大鼠血清HDL的水平均无明显改变。与空白对照组比较,高脂对照组、18.40μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组和1.84μg/kg亚硒酸钠+3.50mg/kg核黄素干预组大鼠血清ALT的水平以及高脂对照组、18.40μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组和18.40μg/kg亚硒酸钠+3.50 mg/kg核黄素干预组大鼠血清AST的水平均升高,差异有统计学意义(P<0.05)。与高脂对照组相比,仅1.84μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组大鼠血清ALT的水平降低,差异有统计学意义(P<0.05);1.84μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组、18.40μg/kg亚硒酸钠+0.70 mg/kg核黄素干预组以及1.84μg/kg亚硒酸钠+3.50 mg/kg核黄素干预组大鼠血清AST的水平降低,差异均有统计学意义(P<0.05)。结论适量亚硒酸钠与核黄素联合暴露可以拮抗高脂饮食所致雄性大鼠血脂及血清肝生化指标的升高。
Objective To study the effects of sodium selenite and riboflavin exposure on serum lipid and serum biochemical markers in male rats with high fat diet. Methods Sixty healthy SPF male SD rats were randomly divided into blank control group (n = 10) and high fat diet group (n = 50) according to their body weight. The rats were given basal diet and high fat diet respectively. After 4 weeks of feeding, The rats in the lipid group were randomly divided into five groups according to their blood lipid levels and body weights: high fat control group, 1.84 μg / kg sodium selenite + 0.70 mg / kg riboflavin intervention group, 18.40 μg / kg sodium selenite + 0.70 mg / kg riboflavin intervention group, 1.84μg / kg sodium selenite +3.50 mg / kg riboflavin intervention group and 18.40μg / kg sodium selenite +3.50 mg / kg riboflavin intervention group, each group 10 only At the same time, the blank control group and high-fat control group were given the same volume of saline, the exposure capacity of 10 ml / kg, once a day for 60 days. The serum levels of total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C) and high density lipoprotein (HDL-C). At the 60th day, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were measured. Results Compared with the blank control group, the serum levels of TC, TG and LDL-C in the high-fat control group and each intervention group were increased during the treatment. Except for the 1.84 μg / kg sodium selenite + 0.70 mg / kg riboflavin intervention group serum TG levels, the difference was statistically significant (P <0.05); the serum HDL-C levels were not significantly changed. Compared with the high-fat control group, the levels of TC and TG in 1.84μg / kg sodium selenite + 0.70mg / kg riboflavin intervention group on the 20th and 40th days and the serum sodium selenite 1.84μg / kg +3.50 TG and LDL-C levels in serum of rats treated with 1 mg / kg riboflavin and 1.84 μg / kg sodium selenite + 0.70 mg / kg riboflavin intervention on day 60 and 18.40 The levels of TC in serum of the rats treated with μg / kg sodium selenite + 0.70 mg / kg riboflavin decreased significantly (P <0.05), while the levels of serum HDL in each intervention group had no significant difference change. Compared with the blank control group, the rats in the high-fat control group, 18.40μg / kg sodium selenite + 0.70 mg / kg riboflavin intervention group and 1.84μg / kg sodium selenite + 3.50mg / kg riboflavin intervention group Serum ALT levels and high-fat control group, 18.40μg / kg sodium selenite + 0.70 mg / kg riboflavin intervention group and 18.40μg / kg sodium selenite +3.50 mg / kg riboflavin intervention serum of rats AST levels were increased, the difference was statistically significant (P <0.05). Compared with the high-fat control group, the serum ALT levels of the rats treated with only 1.84μg / kg sodium selenite + 0.70 mg / kg riboflavin decreased significantly (P <0.05); 1.84μg / kg Sodium selenite + 0.70 mg / kg riboflavin intervention group, 18.40 μg / kg sodium selenite + 0.70 mg / kg riboflavin intervention group and 1.84 μg / kg sodium selenite +3.50 mg / kg riboflavin The level of serum AST decreased in the intervention group, the difference was statistically significant (P <0.05). Conclusion The combination of proper amount of sodium selenite and riboflavin can antagonize the increase of serum lipid and serum biochemical indexes in male rats induced by high-fat diet.