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目的:探讨墓头回提取液对小鼠宫颈癌U14细胞株体外生长的影响。方法:以不同药物浓度和不同时间作用于宫颈癌U14细胞株后,采用MTT法检测其对细胞增殖的影响,并通过倒置显微镜观察细胞形态学变化。结果:墓头回提取液对宫颈癌U14细胞有一定的抑制作用,其抑制率与药物浓度、作用时间有关系;倒置显微镜观察细胞胞体变长,颗粒感增强,细胞浆减少,细胞质减少,细胞脱落呈悬浮状,可见细胞碎片。结论:墓头回提取液可抑制小鼠宫颈癌U14细胞株的增殖。
Objective: To investigate the effect of tomb extract on the growth of mouse U14 cell line in vitro. Methods: U14 cells were treated with different concentrations and different time. The cell proliferation was detected by MTT assay. The cell morphological changes were observed by inverted microscope. Results: The extract of Tomb of the head back to the U14 cells of cervical cancer have a certain degree of inhibition, the inhibition rate and drug concentration, the role of time; inverted microscope observation of cell somatosensory lengthening, increased granularity, decreased cytoplasm, reduced cytoplasm, cells Fall off was suspended, visible cell debris. Conclusion: The tomb extract can inhibit the proliferation of mouse U14 cell line.