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目的观察阳和汤对自身免疫性甲状腺炎模型大鼠甲状腺组织结构及甲状腺自身抗体的影响。方法选取Lewis大鼠30只,正常组10只,其余20只大鼠采用高碘饮食联合猪甲状腺球蛋白皮下注射的方法建立自身免疫性甲状腺炎动物模型,造模成功大鼠分为模型组和中药组,干预8周后采用放免法检测大鼠甲状腺功能及甲状腺球蛋白抗体(thyroglobulin antibody,TGAb),采用酶联免疫吸附测定检测甲状腺过氧化物酶抗体(thyroid peroxidase antibody,TPOAb),甲状腺组织石蜡包埋切片,HE染色。结果与正常组相比,模型组及中药组大鼠促甲状腺激素(TSH)明显降低,血清游离甲状腺素(FT_4)及TPOAb、TGAb明显升高,均具有统计学意义(P<0.01);与模型组大鼠相比,中药组大鼠TPOAb、TGAb降低,有统计学意义(P<0.01);中药组大鼠TPOAb治疗前后差值,与正常组及模型组相比均具有明显差异(P<0.01);中药组大鼠甲状腺组织切片与模型组相比,淋巴细胞浸润程度及甲状腺滤泡细胞破坏均减少。结论阳和汤可降低自身免疫性甲状腺炎大鼠甲状腺自身抗体,减少甲状腺组织淋巴细胞浸润程度,保护甲状腺滤泡细胞等作用。
Objective To observe the effects of Yanghe decoction on thyroid tissue and thyroid autoantibodies in autoimmune thyroiditis model rats. Methods Thirty Lewis rats and 10 normal rats were selected. The remaining 20 rats were induced by autoimmune thyroiditis by subcutaneous injection of high iodine diet combined with porcine thyroglobulin. The rats were divided into model group and control group In the traditional Chinese medicine group, thyroglobulin and thyroglobulin antibody (TGAb) were detected by radioimmunoassay after 8 weeks of intervention. Thyroid peroxidase antibody (TPOAb) and thyroid tissue were detected by enzyme linked immunosorbent assay (ELISA) Paraffin-embedded sections were stained with HE. Results Compared with the normal group, the levels of thyroid stimulating hormone (TSH), serum free thyroxine (FT_4), TPOAb and TGAb in model group and traditional Chinese medicine group were significantly increased (all P <0.01) The TPOAb and TGAb in the TCM group were significantly lower than those in the untreated group (P <0.01). The differences of the TPOAb group before and after treatment in the TCM group were significantly different from those in the normal group and the model group (P <0.01). Compared with the model group, the degree of lymphocytic infiltration and the destruction of thyroid follicular cells in the thyroid tissue of rats in TCM group decreased. Conclusion Yanghe decoction can reduce thyroid autoantibodies in autoimmune thyroiditis rats, reduce the degree of thyroid lymphocyte infiltration and protect thyroid follicular cells.