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目的:观察急性束缚应激和慢性束缚应激对小鼠情绪和学习记忆能力的影响。方法:小鼠按体重和自发活动随机分成三组,即空白对照组,急性应激组和慢性应激组。采用束缚躯体的方法建立急慢性应激模型,并用自发活动实验检测小鼠的情绪状态,避暗实验检测小鼠的学习记忆能力。结果:自发活动实验中急性束缚应激组小鼠平均速度与空白组相比有显著增加(P<0.01),中央区活动时间和中央区活动路程百分比则显著增加(P<0.05),慢性应激组平均速度与空白组相比有显著减小(P<0.01),中央区活动时间和中央区活动路程百分比显著减小(P<0.05);避暗测试中急性应激组避暗潜伏期和错误次数没有改变,而慢性应激组潜伏期显著减小(P<0.05)、错误次数急显著增加(P<0.01)。结论:急性束缚应激会使小鼠产生烦躁情绪、但不会改变其学习记忆能力;慢性束缚应激会使小鼠产生抑郁样情绪,会损害其学习记忆能力。
Objective: To observe the effects of acute restraint stress and chronic restraint stress on emotion and learning and memory in mice. Methods: The mice were randomly divided into three groups according to body weight and spontaneous activity: blank control group, acute stress group and chronic stress group. Binding body was used to establish acute and chronic stress model, and the spontaneous activity test was used to test the emotional state of mice, avoiding the experiment to detect the learning and memory ability of mice. Results: In spontaneous activity test, the average speed of mice in acute restraint stress group increased significantly (P <0.01), the activity time in central area and the percentage of central movement distance increased significantly (P <0.05) Compared with the blank group, the mean velocity of the shock group was significantly decreased (P <0.01), and the activity time in the central region and the percentage of the central movement distance were significantly decreased (P <0.05) The number of errors did not change, while the latency of chronic stress group was significantly reduced (P <0.05), the number of errors was significantly increased (P <0.01). CONCLUSION: Acute restraint stress can cause mice irritability, but not change their ability of learning and memory. Chronic restraint stress can induce depression-like emotion and impair learning and memory ability of mice.