印度农业研究所应用分子标记辅助回交育种技术,开发了一种富含维生素A的杂交玉米。通过使用这种技术,研究人员将β-胡萝卜素羟化酶基因(crtRB1)的等位基因从β-胡萝卜素含量高的自交系导入选定的玉米亲本中。结果表明,在两代回交后,估计有90%后代的亲本基因组得到恢复。此外,在crtRb1基因导入的自交系β-胡萝卜素含量增加了8.6~17.5μg/g。原来的杂交品种中子粒的β-胡萝卜素含量 Indian Institute of Agriculture applied molecular marker-assisted backcross breeding technology to develop a vitamin A-rich hybrid corn. Using this technique, researchers introduced the allele of the β-carotene hydroxylase gene (crtRB1) from selected inbred lines with high β-carotene content into selected maize parents. The results showed that after two generations backcrossing, it is estimated that 90% of the offspring’s parental genome was recovered. In addition, the β-carotene content of inbred lines introduced with crtRb1 increased by 8.6-17.5 μg / g. The β-carotene content of the original hybrid seeds