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通过单克隆抗体测定表明,某些静脉注射丙种球蛋白(IVIG)制备工艺影响IgG生物学活性,其使IgG的亚基有所改变,Fc段也有损害。而完整的Fc和Fab段及其活性在IgG用于免疫缺损病人的替代治疗时十分重要。作者用Opsonistation法检测不同的非脂裹性流感病毒,用ELISA法测定抗H-流感病毒的IgG,结果发现用低pH/微量胃酶处理的IgG优于降解/烷化或β-丙内脂处理的IgG,由于流感抗体广泛存在于正常人群中,因此所有IVIG原料应含有等量抗体,但是对4种IVIG制品的重复检测表明低pH/
Monoclonal antibody assays showed that certain intravenous gamma globulin (IVIG) preparation processes affected IgG biological activity, which altered the subunits of IgG and the Fc segment. However, intact Fc and Fab fragments and their activities are important when IgG is used in the replacement therapy of immunodeficient patients. The authors tested different non-liposomal influenza viruses using the Opsonistation assay and anti-H-influenza virus IgG by ELISA, and found that IgG treated with low pH / microbiase enzyme was superior to degradation / alkylation or beta-propiolactone Treated IgG, since the flu antibodies are widely present in the general population, all IVIG stocks should contain equal amounts of antibodies, but repeated tests on four IVIG preparations showed that low pH /