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目的探讨自然杀伤细胞(NK)在急性肝衰竭进展过程中的作用。方法 40只Balb/c小鼠随机分为实验组和对照组,实验组腹腔注射四氯化碳诱导急性肝衰竭,对照组则注射橄榄油。分离小鼠外周血、肝脏、脾脏的淋巴细胞,用流式细胞仪检测其NK细胞的比例及相关受体表达情况。结果急性肝衰竭小鼠肝脏NK细胞比例较对照组明显升高,差异有统计学意义[(37.2±2.8)%vs(15.3±1.5)%,P=0.0004],外周血以及脾脏中NK细胞比例均较对照组下降。急性肝衰竭小鼠肝脏活化性受体NKP46较抑制性受体NKG2A表达升高更明显,差异有统计学意义[(18.7±1.5)%vs(4.9±2.3)%;t=3.95,P=0.016)]。结论肝脏局部NK细胞在药物诱导的急性肝衰竭中发挥重要的作用。
Objective To investigate the role of natural killer (NK) cells in the progression of acute liver failure. Methods Forty Balb / c mice were randomly divided into experimental group and control group. CCl4-induced acute liver failure was induced in experimental group while olive oil was injected in control group. Lymphocytes were isolated from peripheral blood, liver and spleen of mice, and the proportion of NK cells and the expression of related receptors were detected by flow cytometry. Results The percentage of liver NK cells in acute liver failure mice was significantly higher than that in control group [(37.2 ± 2.8)% vs (15.3 ± 1.5)%, P = 0.0004]. The proportion of NK cells in peripheral blood and spleen Compared with the control group decreased. The expression of NKG2A, an active receptor of liver in acute liver failure mice, was significantly higher than that of the inhibitory receptor NKG2A ([(18.7 ± 1.5)% vs (4.9 ± 2.3)%]; t = 3.95, P = 0.016 )]. Conclusion Local liver NK cells play an important role in drug-induced acute liver failure.