Malaria remains as one of the significant health threat to people living in countries throughout tropical and subtropical zones.Proteomic studies of Plasmodium,the protozoan causing malaria,is essential for understanding its cellular structure,growth stage-specific expression of protein metabolites and complex interaction with host.In-depth knowledge of the pathogen is required for identification of novel biomarkers that can be utilized to develop diagnostic tests and therapeutic antimalarial drugs.The alarming rise in drug-resistant strains of Plasmodium has created an urgent need to identify new targets for drug development that can act by obstructing life cycle of this parasite.In the present review,we briefly discuss on role of various biomarkers including Plnsmodium-assaciated aldolase,histidine-rich proteins and lactate dehydrogenase for diagnosis of malaria.Here we also summarize the present and future prospects of currently used techniques in proteomic approaches such as two dimensional gel electrophoresis and matrix-assisted laser desorption/ionization-time of flight(MALDI-TOF)for diagnosis and potential identification of novel proteins for malaria research. Malaria remains as one of the significant health threats to people living in countries throughout tropical and subtropical zones. Proteomic studies of Plasmodium, the protozoan causing malaria, is essential for understanding its cellular structure, growth stage-specific expression of protein metabolites and complex interaction with host.In-depth knowledge of the pathogen is required for identification of novel biomarkers that can be utilized to develop diagnostic tests and therapeutic antimalarial drugs. The alarming rise in drug-resistant strain of Plasmodium has created an urgent need to identify new targets for drug development that can act by obstructing life cycle of this parasite. In the present review, we briefly discuss on role of various biomarkers including Plnsmodium-assaciated aldolase, histidine-rich proteins and lactate dehydrogenase for diagnosis of malaria. Here we also summarize the present and future prospects of currently used techniques in proteomic approaches such as two di mensional gel electrophoresis and matrix-assisted laser desorption / ionization-time of flight (MALDI-TOF) for diagnosis and potential identification of novel proteins for malaria research.