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目的 :体外测定抗生素S632A3 抗肠道病毒及单纯疱疹病毒I型的作用 ,为筛选新型抗病毒药物提供参考依据。方法 :用不同稀释度的抗生素S632A3 抑制3种肠道病毒及单纯疱疹病毒I型对Vero细胞的感染作用 ,48h后观察细胞病变效应 ,并用MTT法测定细胞活性。结果 :抗生素S632A3 能明显抑制肠道病毒及疱疹病毒对Vero细胞的致病变作用 ,使细胞存活率升高。结论 :该药物有较显著的抗肠道病毒及单纯疱疹病毒I型的作用 ,且对细胞的毒性很低 ,是一种具有潜在开发前景的药物。
OBJECTIVE: To determine the effect of antibiotic S632A3 against enterovirus and herpes simplex virus I in vitro and to provide a reference for screening novel antiviral drugs. Methods: Infection of Vero cells with three kinds of enterovirus and herpes simplex virus type I by S632A3 with different dilutions was performed. After 48 hours, the effect of cytopathic effect was observed. The cell viability was measured by MTT assay. Results: The antibiotic S632A3 could significantly inhibit the pathogenicity of Vero cells by enterovirus and herpesvirus, and increase the cell survival rate. Conclusion: The drug has a significant anti-enterovirus and herpes simplex virus type I effect, and the toxicity of the cell is very low, is a potentially promising drug.