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目的观察紫外线对系统性红斑狼疮(SLE)患者T淋巴细胞Fas抗原的影响。方法将30例SLE患者分为活动期组(A)和非活动期组(B),另设健康对照组(C)15人。采静脉血进行淋巴细胞分离及培养(其中一个培养板进行紫外线照射)后,用双标荧光法分析并获得CD+Fas+细胞百分数。结果在未经紫外线处理的A组Fas表达均显著高于B组(q=9.45,P<0.01)和C组(q=10.71,P<0.01)。B组及C组之间差异无统计学意义(q=1.10,P>0.05);而在经紫外线处理后的A组及B组Fas表达均明显上调(P<0.01)。同时,三组之间q检验显示q值分别为17.29,9.64和7.53,P值均小于0.01,说明均有统计学意义。结论SLE患者外周血T淋巴细胞在紫外线的刺激下更敏感地上调Fas抗原的表达。因此,很可能通过此途径进一步促进T淋巴细胞凋亡。
Objective To observe the effects of ultraviolet light on Fas antigen of T lymphocytes in patients with systemic lupus erythematosus (SLE). Methods Thirty SLE patients were divided into active group (A) and inactive group (B), and another 15 healthy control group (C). After venous blood was collected for lymphocyte isolation and culture (one of the plates was irradiated with UV light), the percentage of CD + Fas + cells was analyzed by double-labeled fluorescence method. Results The expression of Fas in group A without UV treatment was significantly higher than that in group B (q = 9.45, P <0.01) and group C (q = 10.71, P <0.01). There was no significant difference between group B and group C (q = 1.10, P> 0.05). However, the expression of Fas in group A and group B after UV treatment was significantly increased (P <0.01). At the same time, q test between the three groups showed that the q values were 17.29, 9.64 and 7.53 respectively, P values were less than 0.01, indicating that all were statistically significant. Conclusion T lymphocytes in peripheral blood of patients with SLE are more sensitive to the up-regulation of Fas antigen expression under the stimulation of ultraviolet light. Therefore, it is possible to further promote T lymphocyte apoptosis through this pathway.