[Objective]To optimize the separation condition of triterpenoids from Ganoderma lucidum.[Method]The effects of different extraction solvents,different mobile phases and different sampling volume on the separation of triterpenoids were studied by RP-HPLC,and the triterpenoids of 4 kinds of health care products were determined under the optimum condition.[Result]The optimum condition was as follows:extraction solvents of chloroform,mobile phase of 0.2% acetic acid-water,sampling volume of 10-30 μl,and the chromatogram of 2 kinds of the health care products were similar with that of Ganoderma lucidum medicinal material.[Conclusion]The optimum conditions were obtained,and the 4 kinds of health care products were determined by this method,which could used to control the extraction quality of Ganoderma lucidum and evaluate the health care products. [Objective] To optimize the separation condition of triterpenoids from Ganoderma lucidum. [Method] The effects of different extraction solvents, different mobile phases and different sampling volume on the separation of triterpenoids were studied by RP-HPLC, and the triterpenoids of 4 kinds of of [Result] The optimum condition was as follows: extraction solvents of chloroform, mobile phase of 0.2% acetic acid-water, sampling volume of 10-30 μl, and the chromatogram of 2 kinds of the health care products were similar to that of Ganoderma lucidum medicinal material. [Conclusion] The optimum conditions were obtained, and the 4 kinds of health care products were determined by this method, which could used to control the extraction quality of Ganoderma lucidum and evaluate the health care products.