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改良的邻—甲苯胺试剂:取硫脲1克溶于250毫升冰醋酸内,加入邻—甲苯胺50毫升,加丙烯二醇至1000毫升,再加浓硫酸1.5毫升,混合后贮于棕色瓶内,放3℃保存。方法:手指采血后,取20微升血清或血浆于一试管中,加改良的试剂3毫升,混合,于沸水浴中煮10分钟,取出10分钟后,置冰水内冷却。一小时内,在分光光度计上比色,用590毫微米波长调整空白管为零,读取待测管及标准管的光密度。对总蛋白为7.9克％的血清标本进行测定,结果与原法无显著差异。右旋糖酐可使检样变浊而使吸收的读数增高。然而,用本法测定结果得出,大至2克％
Improved o-toluidine Reagent: Take 1 g of thiourea dissolved in 250 ml of glacial acetic acid, add 50 ml of o-toluidine, add propylene glycol to 1000 ml, add 1.5 ml of concentrated sulfuric acid, mix and store in brown bottle Inside, put 3 ℃ save. Methods: After finger blood sampling, take 20 microliters of serum or plasma in a test tube, add 3 ml of modified reagent, mix, boil for 10 minutes in boiling water bath, remove for 10 minutes and cool in ice water. Within one hour, colorimetrically on a spectrophotometer, adjust the blank tube to zero with a wavelength of 590 nm and read the optical density of the tube to be tested and the standard tube. Serum samples of 7.9 grams of total protein were measured, the results were no significant difference with the original method. Dextran can make the sample turbid and absorb the readings increased. However, the results of this method show that as much as 2 g%