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利用分子标记辅助选择将广谱高抗稻瘟病的Pi9(t)基因和全生育期高抗白叶枯病的Xa23基因聚合到同一优良株系中,获得了含双基因的优良株系L10～L13。用来自不同地区的20个稻瘟病小种和中国流行的7个白叶枯病小种及安徽白叶枯病小种对聚合株系进行接菌鉴定,结果显示:聚合Pi9(t)和Xa23基因的株系L10～L13同时抗稻瘟病和白叶枯病;与稻瘟病的供体亲本75-1-127相比,抗性水平相当,均达抗级(R)水平,且抗谱相同;与白叶枯病的供体CBB23相比,对白叶枯病的抗性时期一致,均表现为全生育期抗性,而且抗性水平和抗谱相似。通过田间农艺性状的筛选,获得的双基因聚合系具有较好的农艺性状,可直接应用于生产或作为抗性亲本。
Using molecular marker-assisted selection, the Pi9 (t) gene of broad-spectrum high blast resistance and the Xa23 gene of high resistance to bacterial leaf blight during the whole growth period were polymerized into the same excellent line, and the excellent lines containing double genes L10 ~ L13. The identification of the inoculated strains with 20 rice blast isolates from different regions and 7 popular bacterial blight isolates from China and small race of Anhui bacterial blight showed that the aggregations of Pi9 (t) and Xa23 The resistant lines L10 ~ L13 were resistant to rice blast and bacterial leaf blight at the same time. Compared with the donor parent 75-1-127, Compared with CBB23, the resistance to bacterial blight was consistent during the whole growth period, and the resistance level was similar to that of CBB23. Through the selection of agronomic traits in field, the obtained double-gene polymerization system has good agronomic traits and can be applied directly to production or as a resistant parent.