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酶的催化作用早为人类所熟知和利用。但进一步探讨酶的机理,酶的结构与功能的关系,则需要动力学提供数据,为了最大限度地发挥酶反应的高效率和酶在代谢过程中的作用,以及某些药物的机理等,都需要掌握酶促反应速度的规律。因此深入研讨酶的动力学,引起人们广泛注意,并不断取得进展。本世纪初,Michaelis 与Menten 作了大量实验,在取得足够数据之后,依循“中间产物”假说,1913年提出酶促反应动力学基本原理,并将底物浓度与反应速度的定量关系归纳为一个数学公式,即米氏方程:
Enzymatic catalysis is well known and used by humans. But to further explore the mechanism of the enzyme, the relationship between the structure and function of the enzyme, you need kinetics to provide data, in order to maximize the efficiency of the enzyme reaction and enzyme in the metabolic process, as well as the mechanism of some drugs, etc. Need to grasp the law of the enzymatic reaction rate. Therefore, in-depth study of enzyme kinetics has aroused widespread attention and continued to make progress. At the beginning of this century, Michaelis and Menten made a lot of experiments. After obtaining enough data, following the “intermediate product” hypothesis, the basic principle of enzymatic reaction kinetics was proposed in 1913 and the quantitative relationship between substrate concentration and reaction rate was summarized as one Mathematical formula, the Mie equation: