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目的:观察吡格列酮(Pio)对高脂喂养大鼠骨骼肌组织脂质异位沉积的影响。方法:30只Wistar大鼠平均分为对照组、胰岛素抵抗组和吡格列酮干预组,对照组给予基础饲料,其余2组均给予高脂饲料喂养,其中吡格列酮干预组同时给予吡格列酮20 mg·kg-1·d-1灌胃,各组均喂养16周后检测各组大鼠血游离脂肪酸(FFA)水平及骨骼肌组织中三酰甘油(TG)含量,同时应用蛋白免疫印迹法检测骨骼肌组织一磷酸腺苷活化蛋白激酶α(AMPKα)磷酸化水平。结果:与对照组比较,胰岛素抵抗组大鼠胰岛素敏感性显著降低,血FFA水平及骨骼肌组织TG含量增高,骨骼肌组织中AMPKα磷酸化水平降至对照组水平的52.9%;与胰岛素抵抗组比较,吡格列酮干预组大鼠胰岛素敏感性明显提高,血FFA水平及骨骼肌组织TG含量显著下降,骨骼肌组织中AMPKα磷酸化水平显著提高至对照组的81.3%。但是与对照组比较,吡格列酮干预组大鼠血FFA水平及骨骼肌组织TG含量仍然较高,而且胰岛素敏感性及骨骼肌组织中AMPKα磷酸化水平仍然显著降低。结论:高脂饮食诱导胰岛素抵抗,吡格列酮干预可以通过降低血FFA水平及减轻骨骼肌组织脂质异位沉积改善其胰岛素抵抗。
Objective: To observe the effect of pioglitazone on lipid metabolism in skeletal muscle of high fat diet rats. Methods: Thirty Wistar rats were equally divided into control group, insulin resistance group and pioglitazone intervention group. The control group was given basic diet and the other two groups were fed with high-fat diet. Pioglitazone intervention group was given pioglitazone 20 mg · kg-1 · D-1 were fed into the stomach. After 16 weeks of feeding, the levels of free fatty acids (FFA) and the content of triglyceride (TG) in skeletal muscle of rats in each group were measured. Western blotting Phosphorylation of adenosine triphosphate activates protein kinase alpha (AMPKα) phosphorylation. Results: Compared with the control group, the insulin sensitivity of the insulin resistance group was significantly decreased, the blood FFA level and TG content of skeletal muscle increased, and the level of AMPKα phosphorylation in skeletal muscle decreased to 52.9% of the control group. Compared with the insulin resistance group Compared with the control group, the insulin sensitivity of pioglitazone group was significantly increased, the level of blood FFA and the content of TG in skeletal muscle significantly decreased, and the phosphorylation level of AMPKα in skeletal muscle significantly increased to 81.3% of the control group. However, compared with the control group, the blood levels of FFA and skeletal muscle TG in the pioglitazone-treated group were still high, and the insulin sensitivity and the level of AMPKαphosphorylation in skeletal muscle were still significantly decreased. Conclusion: High-fat diet induces insulin resistance. Pioglitazone can improve insulin resistance by decreasing blood FFA levels and alleviating lipid deposition in skeletal muscle.