论文部分内容阅读
以花生根瘤菌高效工程菌株ＨＮ１１，ＨＮ１２和ＨＮ１３各自的增效重组质粒为材料，在共生条件下和非共生人工液体培养基中，研究其在各自宿主快生型花生根瘤菌８５－７和慢生型花生根瘤菌ＣＯ２－５中的稳定性．结果表明：在根瘤内，８５－７宿主中重组质粒与空载体Ｐ（ＬＡＰＲ１）丢失程度相似，与外源片段存在无关；而在ＣＯ２－５宿主中ＨＮ１３的增效重组质粒比无效重组质粒及空载体Ｐ（ＬＡＰＲ１）丢失程度较轻，在人工液体培养基中，Ｐ（ＬＡＰＲ１）及其重组质粒在同一宿主中丢失程度相似．并讨论了以Ｐ（ＬＡＰＲ１）为载体的重组质粒丢失较严重的原因，提出了保持工程菌中重组质粒稳定性的相应措施．
Using the recombinant plasmids of HN11, HN12 and HN13, which are highly efficient strains of rhizobia of peanut, respectively, under the symbiotic conditions and the non-symbiotic artificial liquid medium, the quick-growth peanut rhizobia 85-7 Strain of green peanut rhizobium CO2-5. The results showed that in Nodule, the loss degree of recombinant plasmids in 85-7 host was similar to that of empty vector P (LAPR1), but not in the presence of exogenous fragments. However, in H513 host, The loss of empty vector P (LAPR1) was relatively slight. In artificial liquid medium, P (LAPR1) and its recombinant plasmid lost in the same host to a similar extent. The reasons why the recombinant plasmids with P (LAPR1) as a vector were lost more seriously were discussed. Corresponding measures to maintain the stability of recombinant plasmids in engineering bacteria were proposed.