论文部分内容阅读
目的:研究维生素B12对多发性口腔溃疡(ROU)家兔血清中白介素-6(IL-6)、白介素-8(IL-8)表达水平的影响。方法:将40只家兔随机分为4组,每组10只,随机选取10只设立空白对照组,其余30只用免疫法建立ROU家兔模型,然后随机分3组,分别为阳性对照组、左旋咪唑组及维生素B12组。观察口腔溃疡的变化,采用酶联免疫吸附法检测各组家兔灌胃结束后血清中IL-6、IL-8的含量。结果:阳性对照组与空白对照组比较,阳性对照组中IL-6、IL-8含量明显升高,差异具有统计学意义(P<0.01);左旋咪唑组和维生素B12组与阳性对照组比较,两实验组中的IL-6、IL-8水平明显低于阳性对照组,差异具有统计学意义(P<0.01);维生素B12组与左旋咪唑组相比,差异无统计学意义。结论:维生素B12通过补充微量元素,调节机体免疫功能,调控机体中细胞因子的表达,显著降低血清中IL-6、IL-8的高水平含量,这可能为其治疗ROU的作用机制之一。
Objective: To investigate the effect of vitamin B12 on the expression of interleukin-6 (IL-6) and interleukin-8 (IL-8) in rabbits with multiple oral ulcers (ROU). Methods: Forty rabbits were randomly divided into 4 groups with 10 mice in each group. Ten rabbits were randomly selected to establish a blank control group. The remaining 30 rabbits were randomly divided into 3 groups: the positive control group , Levamisole group and vitamin B12 group. The changes of oral ulcer were observed. The levels of IL-6 and IL-8 in sera of rabbits in each group were detected by enzyme-linked immunosorbent assay (ELISA). Results: Compared with the blank control group, the content of IL-6 and IL-8 in the positive control group was significantly increased (P <0.01); the levamisole and vitamin B12 groups compared with the positive control group . The levels of IL-6 and IL-8 in the two experimental groups were significantly lower than those in the positive control group (P <0.01). There was no significant difference between the vitamin B12 group and the levamisole group. CONCLUSION: Vitamin B12 can regulate the immune function, regulate the expression of cytokines and significantly reduce the high levels of IL-6 and IL-8 in serum, which may be one of the mechanisms of action of vitamin B12 in the treatment of ROU.