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目的 :探讨丹参有效成分丹酚酸B在心脏微血管内皮细胞 (CMEC)缺氧 /复氧损伤中的保护作用。方法 :通过心脏微血管内皮细胞体外培养技术 ,建立缺氧预适应 (HPC)模型并给予丹酚酸B ,应用酶联免疫方法检测细胞间粘附分子 1(ICAM 1)表达。结果 :在CMEC缺氧 3h ,复氧 6h后ICAM 1表达升高 ;缺氧预适应和应用丹酚酸B对CMEC进行预处理则能有效降低CMECICAM 1的表达。结论 :丹酚酸B在CMEC缺氧 /复氧损伤中可产生与缺氧预适应类似的保护效果。
Objective: To investigate the protective effect of salvianolic acid B, an effective component of Salvia miltiorrhiza, on hypoxia/reoxygenation injury in cardiac microvascular endothelial cells (CMEC). METHODS: The hypoxic preconditioning (HPC) model was established by in vitro culture of cardiac microvascular endothelial cells and salvianolic acid B was administered. The expression of intercellular adhesion molecule 1 (ICAM 1) was detected by enzyme-linked immunosorbent assay. RESULTS : The expression of ICAM 1 increased after hypoxia 3h and reoxygenation 6h in CMEC. Hypoxic preconditioning and pretreatment of CMEC with salvianolic acid B could effectively reduce the expression of CMECICAM 1. CONCLUSION : Salvianolic acid B can produce protective effects similar to hypoxic preconditioning in anoxia/reoxygenation injury of CMEC.