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目的探讨结核杆菌聚合酶链反应(PCR)在肺外结核病中早期诊断的临床意义。方法收集134例标本,分别用抗酸染色法、培养法、PCR法对结核杆菌进行检测比较。结果抗酸染色法与PCR法比较阳性率分别为23.4%、53.2%;培养法与PCR法比较阳性率分别为28.3%、47.8%,两组比较差异有统计学意义(P<0.05)。PCR法阳性率比抗酸染色法、培养法分别高出了29.8%、19.5%;并且抗酸染色法和培养法阳性的标本PCR法100%阳性,对照组PCR法100%阴性。结论 PCR法对结核杆菌的检测具有高特异、高敏感、快速、简便等特点,在肺外结核的早期诊断中具有很好的诊断参考价值。
Objective To investigate the clinical significance of early diagnosis of tuberculosis in tuberculosis by polymerase chain reaction (PCR) of Mycobacterium tuberculosis. Methods Totally 134 specimens were collected and tested by acid-fast staining, culture and PCR respectively. Results The positive rates of acid-fast staining and PCR were 23.4% and 53.2%, respectively. The positive rates of culture and PCR were 28.3% and 47.8%, respectively. There was significant difference between the two groups (P <0.05). The positive rate of PCR was 29.8% and 19.5% higher than that of acid-fast staining and culture methods respectively. The positive rate of PCR-positive and acid-fast staining were 100% positive and 100% of the negative control by PCR. Conclusion PCR method has the characteristics of high specificity, high sensitivity, quickness and convenience for the detection of Mycobacterium tuberculosis. It has a good diagnostic value in the early diagnosis of extrapulmonary tuberculosis.