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在石蜡切片制作过程中，经福尔马林固定、脱水、包埋标本以后，组织中的抗原被这或由于蜡块存放时间过长、放置时的温度变化又较大，而致使抗原丢失及其敏感度降低。若用石蜡切片做免疫组化检测，常因以上原因，导致染色效果欠佳，甚至失败。在免疫组化PAP法中，我们选择石蜡切片用微波处理，进行抗原修复，把被遮蔽的抗原暴露出来，提高反应敏感度和染色效果，并可减少反应时间，得到了较为满意的结果。所用微波炉为松下公司产品（Na－tionalNN56525），选择微波火力为-Lo档（75W），能产生92－98C温度。材料选用经Zamboni’s固定液灌注固定的大鼠甲状腺，乙醇系列脱水，石蜡包埋后放置两年的切片（7Pm），二甲醛脱蜡至水。具体方法如下：0．01MPBS漂洗3×5’→9：1甲醇-H2O2处理10Min→001MPBS2×5’→0．01M柠檬酸盐缓冲液浸泡并用微波炉“Lo”档加热9min→冷却后0．01MPBS漂洗2×5’→l％BSA37℃30min→一抗（CT）37℃2h、室温过夜（12h）-PBS漂洗2×5’→二抗（GAR）37Clh→PBS漂洗2×5’～PAP37℃1h→DAB显色。结果使整个实验过程比常规免疫组化PAP法缩短近乎30小时，并省略了胰酶消化步骤，达到了更好的染色效果。
In the process of paraffin sectioning, after fixing, dehydration and embedding of the specimen in formalin, the antigen in the tissue is either caused by this or because the wax block is stored for too long and the temperature change during placement is relatively large, resulting in the loss of antigen and Its sensitivity is reduced. If paraffin sections do immunohistochemistry, often due to the above reasons, resulting in poor staining, or even failure. In the immunohistochemical PAP method, we selected the paraffin sections by microwave treatment, antigen retrieval, the exposed antigen is exposed to improve the reaction sensitivity and staining effects, and can reduce the reaction time and get more satisfactory results. Microwave oven used for Panasonic products (Na-tionalNN56525), select the microwave power -Lo file (75W), can produce 92-98C temperature. MATERIALS: The fixed thyroid gland was infused with Zamboni’s fixative solution. The ethanol series was dehydrated and placed in paraffin for two years after slicing (7 Pm). The formaldehyde was dewaxed to water. The specific method is as follows: 0.01MPBS rinse 3 × 5 ’→ 9: 1 methanol -H2O2 treatment 10Min → 001MPBS2 × 5’ → 0.01M citrate buffer soaking and microwave oven “Lo” file heating 9min → after cooling 0.01MPBS Rinsing 2 × 5 ’→ 1% BSA at 37 ° C for 30 min → primary antibody (CT) at 37 ° C for 2 h, overnight at room temperature (12 h) -PBS rinse 2 × 5’ → secondary (GAR) 37Clh → PBS rinse 2 × 5 ’ 1h → DAB color. As a result, the whole experimental process was shortened by nearly 30 hours as compared with the conventional immunohistochemical PAP method, and the tryptic digestion step was omitted to achieve a better dyeing effect.