以往我们在豚鼠肺切片制作中采用断头放血取全肺内固定的方法 ,但是 ,结果发现肺泡壁Ⅰ型和Ⅱ型细胞较难分辨 ,肺泡隔比较厚 ,毛细血管不能分辨 ,毛细血管内皮细胞与肺泡壁Ⅰ型细胞也难于分辨 ,肺泡腔内可见有残血污染。现我们将豚鼠肺切片制作方法进行改良 ,即先行气管结扎 , In the past, we used decapitation to take whole lung fixation in the production of guinea pig lung slices. However, it was found that the type I and type II cells in the alveolar wall were more difficult to distinguish. The alveolar septum was thicker and the capillaries could not be distinguished. The capillary endothelial cells And alveolar wall type I cells are difficult to distinguish, alveolar visible residual blood pollution. Now we will guinea pig lung slice making method to be improved, that is, the first tracheal ligation,