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为探讨氟化钠对小鼠睾丸谷胱甘肽抗氧化系统的影响,将24只3周龄ICR雄性小鼠随机分为对照组和染氟组,对照组给予蒸馏水,染氟组分别供给含25、50、100mg·L~(-1) NaF的蒸馏水,自由饮用60d。染毒结束后检测小鼠睾丸总抗氧化能力(T-AOC)、活性氧(ROS)、丙二醛(MDA)、还原型谷胱甘肽(GSH)的含量及谷胱甘肽过氧化氢酶(GPx)、谷胱甘肽硫转移酶(GST)、谷胱甘肽还原酶(GR)的活性,检测GPx1、GPx4、GST-mu5和GR基因的转录。电镜观察睾丸组织细胞内线粒体、内质网和高尔基体的结构。结果显示,氟中毒可使睾丸T-AOC减弱,ROS、MDA含量升高,GSH含量显著降低,细胞内氧化水平升高,线粒体、内质网和高尔基体出现不同程度的损伤,GPx、GST、GR的活性降低,GPx1、GPx4、GST-mu5和GR基因转录下调。小鼠氟中毒后,睾丸抗氧化能力降低,处于明显的氧化应激状态,这与谷胱甘肽抗氧化系统酶活性降低及其相关基因转录下调有关。
In order to investigate the effect of sodium fluoride on glutathione antioxidant system in mice testes, twenty-four ICR male mice of 3 weeks old were randomly divided into control group and fluoride group. Control group was given distilled water, 25,50,100 mg · L -1 NaF of distilled water, free to drink 60d. T-AOC, reactive oxygen species (ROS), malondialdehyde (MDA), reduced glutathione (GSH) and glutathione hydroperoxide (GPx), glutathione S-transferase (GST) and glutathione reductase (GR) activity were measured. The transcription of GPx1, GPx4, GST-mu5 and GR genes was detected. The structure of mitochondria, endoplasmic reticulum and Golgi in testicular cells were observed by electron microscope. The results showed that fluoride poisoning could degrade the T-AOC, increase the content of ROS and MDA, decrease the content of GSH, increase the level of intracellular oxidation and damage the mitochondria, endoplasmic reticulum and Golgi, GR activity decreased, GPx1, GPx4, GST-mu5 and GR gene transcription down-regulation. Fluorosis in mice, testis antioxidant capacity decreased, in a significant oxidative stress, which is related to reduced glutathione antioxidant enzyme activity and related gene transcription down.