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目的:分析精子DNA碎片与体外受精(IVF)结局的相关性。方法:选取东莞市第三人民医院2014至2015年收治的男性患者200例作为研究对象,采用染色质扩散试验的方法进行精子DNA碎片与体外受精的检测,将精子中的DNA碎片率(DFI)、精液基本数据、受精率、可移植胚胎率进行分析,对精子DNA碎片率(DFI)、精子分析得出的各项参数进行回归分析。结果:经分析发现,精子DFI和精子活动率呈现负相关,采用密度梯度法对前期和后期的精子DFI进行分析,发现前后精子DFI和精子DNA碎片与IVF结局的受精率呈现负相关,差异具有统计学意义(P<0.05)。结论:精子DFI能够直接影响精子的活力和精子DNA碎片与IVF结局的受精率,其指标检测能够有效预测精子DNA碎片与IVF结局的受精率。
Objective: To analyze the correlation between sperm DNA fragmentation and in vitro fertilization (IVF) outcome. Methods: A total of 200 male patients from 2014 to 2015 in Dongguan Third People ’s Hospital were enrolled in this study. Chromosome diffusion test was used to detect sperm DNA fragmentation and in vitro fertilization. DNA fragmentation rate (DFI) , Semen basic data, fertilization rate and transferable embryo rate were analyzed. Regression analysis was carried out on the parameters of sperm DNA fragmentation rate (DFI) and sperm analysis. Results: The results showed that there was a negative correlation between sperm motility and DFI. The density gradient method was used to analyze the early and late spermatozoa DFI. The results showed that there was a negative correlation between fertilization rates of DFI and sperm DNA fragments and IVF outcome Statistical significance (P <0.05). Conclusion: Sperm DFI can directly affect sperm motility and fertilization rate of sperm DNA fragmentation and IVF outcome. The index testing can effectively predict the fertilization rate of sperm DNA fragmentation and IVF outcome.