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目的:探讨孕三烯酮(gestrinone)治疗子宫内膜异位症的作用机理。方法:用自体移植术建立大鼠子宫内膜异位症动物模型,随机分组给药,3周后剖腹,取血测定雌二醇(E2)、孕酮(P)浓度,取出子宫及移植物,固定、切片,免疫组化SP法测定各组动物子宫及移植物的雌激素受体(ER)和孕激素受体(PR)的表达。结果:孕三烯酮高剂量使大鼠血清E2、P水平降低,而中低剂量对E2、P水平影响不大。免疫组化结果则显示移植物的ER、PR含量较子宫内膜低,孕三烯酮高、中剂量可下调在位和异位子宫内膜ER、PR表达,而低剂量孕三烯酮则不能明显改变ER、PR的表达量。结论:孕三烯酮可通过降低体内雌孕激素水平以及下调在位及异位子宫内膜ER、PR的表达,发挥对子宫内膜异位症的治疗作用。
Objective: To investigate the mechanism of gestrinone in the treatment of endometriosis. Methods: The rat model of endometriosis was established by autograft. The rats were randomly divided into three groups: cesarean section, blood estradiol (E2) and progesterone (P) were taken to determine the concentration of uterus and uterus The expressions of estrogen receptor (ER) and progesterone receptor (PR) in the uterus and the grafts of each group were determined by immunohistochemical SP method. Results: The high dose of gestrinone decreased serum E2 and P levels in rats, while the low and middle dose of gestrinone had little effect on E2 and P levels. The results of immunohistochemistry showed that the ER and PR contents in the grafts were lower than those in the endometrium and the high and middle doses of gesthenone lowered the expression of ER and PR in the eutopic and ectopic endometrium while the low dose gestrinone Can not significantly change the expression of ER, PR. CONCLUSION: Gestrinone exerts the therapeutic effect on endometriosis by decreasing the levels of estrogen and progesterone in the body and decreasing the expressions of ER and PR in the eutopic and ectopic endometrium.