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目的建立HPLC法测定灯台叶片中灯台树次碱(scholarisine)、19-表灯台树次碱(19-epischolarisine)和瓦莱萨明碱(vallesamine)的含量。方法采用Diamonsil-C_(18)(2)(250 mm×4.6 mm,5μm)色谱柱,流动相:体积分数为0.3%的甲酸水溶液(A),乙腈-甲醇(体积比为:80:20)(B),梯度洗脱,流速:1.0 m L·min~(-1),柱温:30℃,紫外检测波长:285 nm。结果灯台树次碱、19-表灯台树次碱和瓦莱萨明碱的质量浓度分别在3.744~37.44、1.116~11.16、3.840~38.40 mg·L~(-1)内与峰面积呈良好的线性关系(r>0.999 0),平均加样回收率分别为98.9%(RSD=2.2%)、100.9%(RSD=2.6%)和99.9%(RSD=1.5%)(n=6)。结论该方法可用于同时测定灯台叶片中灯台树次碱、19-表灯台树次碱和瓦来萨明碱的含量。
OBJECTIVE To establish a HPLC method for the determination of scholarisine, 19-epischolarisine and vallesamine in lampshade leaves. Methods Diamonsil-C 18 (2) (250 mm × 4.6 mm, 5 μm) column was used. The mobile phase consisted of a 0.3% (B), gradient elution, flow rate: 1.0 m L · min -1, column temperature: 30 ℃, UV detection wavelength: 285 nm. Results The concentrations of mescaline, 19-epicatechin and valerianine in the range of 3.744 ~ 37.44,1.116 ~ 11.16,3.840 ~ 38.40 mg · L ~ (-1) The linear relationship (r> 0.999 0) with an average recovery of 98.9% (RSD = 2.2%), 100.9% (RSD = 2.6%) and 99.9% (RSD = 1.5%), respectively. Conclusion The method can be used for the simultaneous determination of strychnine, 19-epicarbazide and valerianine in the lamp holder.