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本文研究目的是给家兔注射大肠杆菌后,根据全身对该菌的清除及细菌在器官的分布了解内毒素和肿瘤坏死因子(TNF)对免疫功能的潜在影响。为了能使清除过程定量化,设计了4组:(1)静脉内注射大剂量TNF后60min;(2)注射内毒素后1h;(3)注射内毒素后4h;(4)注射盐水后(对照组),再静脉注射已知数量的外源性大肠杆菌,同时对第1～3组作不注大肠杆菌的对照,以排除TNF或内毒素导致内源性大肠杆菌移位的可能。监测的参数为动脉压、氧吸收和细菌从血液的清除力。结果表明,对照组和TNF处理组以及注射内毒素1h组的动物在观察期间,动脉压和氧吸收无明显改变,而注射内毒素4h组动物动脉压和氧吸收降低。对于外源
The aim of this study is to understand the potential effects of endotoxin and tumor necrosis factor (TNF) on immune function based on systemic clearance of the bacteria and distribution of the bacteria in the organism. In order to quantify the clearance process, four groups were designed: (1) intravenous injection of high-dose TNF at 60 min; (2) 1 h after endotoxin injection; (3) 4 h after endotoxin injection; (4) Control group), then intravenous injection of a known amount of exogenous E. coli, while the first 3 groups were not injected E. coli control to rule out TNF or endotoxin cause endogenous Escherichia coli translocation possible. The parameters monitored were arterial pressure, oxygen uptake and bacterial clearance from the blood. The results showed that the arterial pressure and oxygen absorption of the animals in the control group and the TNF-treated group and the endotoxin-1h group did not change significantly during the observation period, while the arterial pressure and oxygen absorption in the 4h group were decreased. For exogenous