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目的 建立大鼠条件性位置偏爱模型(CPP),探讨褪黑素对吗啡依赖大鼠复吸行为的影响。方法连续6d按剂量递增法于大鼠皮下注射吗啡,诱导实验组和吗啡对照组大鼠CPP形成,然后用盐水替代吗啡皮下注射大鼠10d,使CPP逐渐消退后,再用吗啡4mg/kg单次引燃注射激发消退的条件性位置偏爱复燃,3个实验组分别在注射吗啡前30min腹腔注射褪黑素20mg/、40mg/kg和80mg/kg,分别观察各组大鼠行为。结果 经6d吗啡训练后,实验组和吗啡对照组大鼠在伴药箱的停留时间显著延长,吗啡诱导的大鼠CPP形成;停用吗啡后,经10d的生理盐水注射,吗啡诱导的大鼠CPP逐渐消退;吗啡4mg/kg单次引燃注射使大鼠消退的CPP恢复,而实验组可以剂量依赖性地减弱大鼠CPP恢复。结论 吗啡诱导大鼠CPP形成,褪黑素在一定程度抑制吗啡依赖大鼠的复吸行为。
Objective To establish a conditioned place preference model (CPP) in rats and explore the effect of melatonin on relapse behavior in morphine dependent rats. Methods The rats were injected subcutaneously with morphine for 6 days, and CPP was induced in experimental group and morphine control group. Rats were then injected subcutaneously with saline instead of morphine for 10 days. CPP gradually subsided and then morphine 4 mg / kg In the three experimental groups, melatonin 20 mg / kg, 40 mg / kg and 80 mg / kg were injected intraperitoneally 30 min before morphine injection respectively, and the behavior of rats in each group was observed respectively. Results After 6 days of morphine training, the rats in the experimental group and the morphine control group stayed in the cocoon box prolonged significantly, morphine-induced CPP was formed in rats; morphine-induced rats were injected with morphine for 10 days after morphine withdrawal CPP gradually subsided; single injection of 4mg / kg morphine restored CPP recovery rats, and the experimental group can dose-dependently reduce CPP recovery in rats. Conclusion Morphine induced the formation of CPP in rats, and melatonin inhibited the relapse behavior of morphine dependent rats to a certain extent.