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由甲基胆蒽诱发纯系豚鼠两株不同的肿瘤——脂肪肉瘤(MCA-A)和骨肉瘤(MGA-25)。将这两株肿瘤细胞悬液用低频声波处理,130,000g 离心45分钟后得到的抽提液,经真空透析浓缩制备无细胞肿瘤抗原。以 MGA-A 或 MGA-25活
Two different tumors - liposarcoma (MCA-A) and osteosarcoma (MGA-25) - were induced by methylcholanthrene in pure guinea pigs. The two tumor cell suspensions were treated with low-frequency sonication and centrifuged at 130,000g for 45 minutes. The extract was concentrated by vacuum dialysis to prepare a cell-free tumor antigen. Live with MGA-A or MGA-25