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BACKGROUND:Subsequent to cerebral ischemic injury, endogenous neural stem cells are activated, but ischemia-induced neuronal loss is not compensated by ischemic injury-induced neural regeneration.Salvia (S.) miltiorrhiza Bge.f.alba (Baihua Danshen, a Chinese herbal medicine) could enhance learning and memory functions, as well as promote neural regeneration.OBJECTIVE:To observe the effects of S.miltiorrhiza Bge.f.alba on recovery from cerebral ischemia-reperfusion injury, and the influence on neuronal regeneration and differentiation.DESIGN, TIME AND SETTING:Randomized, controlled, animal experiments were performed at the Experimental Animal Center and Neurobiology Laboratory of Taishan Medical College in September of 2006.MATERIALS:S.miltiorrhiza Bge.f.alba was provided by Taishan Medical College Botanic Garden, Taian, China; dl-3n-butylphthalide (NBP) soft capsule was purchased from NBP Pharmaceutical, Shijiazhuang, China; mouse anti-bromodeoxyuridine antibody, rabbit anti-NF200 antibody, and bromodeoxyuridine were purchased from Sigma, Louis, MO, USA; Annexin V-fluorescein isothiocyanate/PI apoptosis kit was purchased from Nanjing Comissariado Biological Technology Development, Nanjing, China.METHODS:Adult Sprague Dawley rats were randomly assigned to sham surgery, model (cerebral ischemia and reperfusion, without administration), S.miltiorrhiza Bge.f.alba, and NBP groups.Following establishment of the cerebral ischemia/reperfusion model, S.miltiorrhiza Bge.f.alba or NBP (1 mL/100 g) was respectively perfused at 30 minutes following cerebral ischemia/reperfusion.MAIN OUTCOME MEASURES:Alterations in cerebral blood flow before and after ischemia/reperfusion, NF200-and bromodeoxyuridine-double positive cells in striatum of affected tissue as well as neuronal apoptosis rate at days 5 and 7 following cerebral ischemia/reperfusion.RESULTS:Subsequent to cerebral ischemia reperfusion, cerebral blood flow was reduced.Following treatment with S.miltiorrhiza Bge.f.alba, cerebral blood flow significantly increased (P<0.05).NBP treatment was inferior to S.miltiorrhiza Bge.f.alba with regard to stabilization of cerebra blood flow (P<0.05).S.miltiorrhiza Bge.f.alba significantly increased the number of newly formed neurons in rats following cerebral ischemia (P<0.05) and significantly reduced neuronal apoptosis (P<0.05), with no significant difference compared with NBP treatment (P>0.05).CONCLUSION:S.miltiorrhiza Bge.f.alba significantly increased cerebral blood flow, reduced neuronal apoptosis, and promoted neuronal regeneration in rats with cerebral ischemia/reperfusion impairment.
BACKGROUND: Subsequent to cerebral ischemic injury, endogenous neural stem cells are activated, but ischemia-induced neuronal loss is not compensated by ischemic injury-induced neural regeneration. Salvia (S.) miltiorrhiza Bge.f. alba (Baihua Danshen, a Chinese herbal medicine) could enhance learning and memory functions, as well as promote ovary regeneration. OBJECTIVE: To observe the effects of S. miltiorrhiza Bge.f. alba on recovery from cerebral ischemia-reperfusion injury, and the influence on neuronal regeneration and differentiation. , TIME AND SETTING: Randomized, controlled, animal experiments were performed at the Experimental Animal Center and Neurobiology Laboratory of Taishan Medical College in September of 2006. Matella S: S. miltiorrhiza Bge.f.alba was provided by Taishan Medical College Botanic Garden, Taian , China; dl-3n-butylphthalide (NBP) soft capsule was purchased from NBP Pharmaceutical, Shijiazhuang, China; mouse anti-bromodeoxyuridine antibody, rabbit anti-NF200 anti Annexin V-fluorescein isothiocyanate / PI apoptosis kit was purchased from Nanjing Comissariado Biological Technology Development, Nanjing, China. METHODS: Adult Sprague Dawley rats were randomly assigned to sham surgery, model (cerebral ischemia and reperfusion, without administration), S. miltiorrhiza Bge.f. alba, and NBP groups. Fluorlowing establishment of the cerebral ischemia / reperfusion model, S. miltiorrhiza Bge.f.alba or NBP (1 mL / 100 g ) were respectively perfused at 30 minutes following cerebral ischemia / reperfusion. MAIN OUTCOME MEASURES: Alterations in cerebral blood flow before and after ischemia / reperfusion, NF200-and bromodeoxyuridine-double positive cells in striatum of affected tissue as well as neuronal apoptosis rate at days 5 and 7 following cerebral ischemia / reperfusion .RESULTS: Subsequent to cerebral ischemia reperfusion, cerebral blood flow was reduced. Popular treatment with S. miltiorrhiza Bge.f. alba, cer(P <0.05) .NBP treatment was inferior to S. miltiorrhiza Bge.f.alba with regard to stabilization of cerebra blood flow (P <0.05). S.miltiorrhiza Bge.f.alba significantly increased the number of newly formed neurons in rats following cerebral ischemia (P <0.05) and significantly reduced neuronal apoptosis (P <0.05), with no significant difference compared with NBP treatment (P> 0.05) .CONCLUSION: S. miltiorrhiza Bge.f.alba increased cerebral blood flow, reduced neuronal apoptosis, and promoted neuronal regeneration in rats with cerebral ischemia / reperfusion impairment.