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目的对临床绝经前诊刮标本采用子宫内膜上皮内瘤变(EIN)标准对其分类及进行相关临床病理分析,同时检测人第10号染色体缺失的磷酸酶及张力蛋白同源基因(PTEN)和B细胞淋巴瘤/白血病-2(bcl-2)蛋白表达,探讨绝经前EIN分子特性及生物学特点。方法收集113例绝经前妇女子宫内膜存档标本,应用EIN标准对其重新分类并采用免疫组化检测PTEN和bcl-2的分子表达情况。结果 EIN的诊断标准与世界卫生组织(WHO,1994年)子宫内膜增生分类标准之间没有固定的对应关系,二者间有区别也有联系。从良性到恶性,出现PTEN和bcl-2缺失率逐渐增加的情况,提示PTEN和bcl-2在子宫内膜样癌发展的过程中有一定作用。但EIN病变中表达与其他组相比差异无统计学意义。结论 EIN病变诊断和预后判断应以组织形态学标准为主,以分子指标PTEN和Bcl-2为辅助判断。
Objective To classify and analyze the clinical and pathological features of clinically premenopausal women by using endometrial intraepithelial neoplasia (EIN) and detect the PTEN (phosphatase and tensin homology gene) And B cell lymphoma / leukemia-2 (bcl-2) protein expression, to explore the molecular characteristics of premenopausal EIN and biological characteristics. Methods Seventy-one samples of endometrium from premenopausal women were collected and reclassified using EIN standard. The expression of PTEN and bcl-2 was detected by immunohistochemistry. Results There was no definite correspondence between the diagnostic criteria of EIN and the classification criteria of endometrial hyperplasia of the World Health Organization (WHO, 1994). From benign to malignant, there is a gradual increase in the deletion rate of PTEN and bcl-2, suggesting that PTEN and bcl-2 play a role in the development of endometrial carcinoma. However, there was no significant difference between EIN group and other groups. Conclusions The diagnosis and prognosis of EIN should be based on the histomorphological criteria and the molecular markers PTEN and Bcl-2 as adjuvant judgments.