论文部分内容阅读
通过白粉病抗性鉴定、生化标记及分子原位杂交相结合的方法 ,从小麦 (TriticumaestivumL .)幼胚培养组合T2 40 (普通小麦×小麦_簇毛麦 (HaynaldiavillosaLam .) 6D/6V异代换系 )的 32个SC2 代株系中 ,筛选出T2 40_6株系 ,其所有的抗白粉病单株均缺失簇毛麦 6V染色体长臂上的谷草转氨酶位点GOT_V2 ,而具有短臂上的醇溶蛋白位点Gli_V2 。细胞学染色体观察表明 ,该株系的所有抗病单株均具有 1～ 2个端体 ,这些端体不能与小麦染色体配对 ,双端体之间可以配对。经原位杂交分析 ,端体杂交呈阳性 ,表明它们均为簇毛麦 6V染色体短臂 (6VS)。
Through the combination of powdery mildew resistance assay, biochemical labeling and molecular in situ hybridization, Triticum aestivum L. hybrid embryos were stained with T240 (Haynaldia villosa Lam. 6D / 6V) Line) of 32 SC2 lines, T240_6 lines were screened out, and all of the powdery mildew resistant plants had GOT_V2 as the aspartate aminotransferase site on the 6V chromosome long arm, Sliver protein site Gli_V2. Cytological chromosome observations showed that all the resistant plants of this strain had 1-2 terminal bodies, which could not be paired with the wheat chromosomes and could be paired between the two terminal bodies. By in situ hybridization analysis, the terminal hybridization was positive, indicating that they are 6V chromosome short arm (6VS).