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表面污染是木本植物组织培养污染率高的主要原因。选择晴、阴天气下生长的试材或者采用茎尖培养,是降低污染率、成功建立无菌初代培养物的有效措施。适合黄皮茎段初代培养的条件为:WPM+BA1.0+KT0.5+NAA0.5+GA_30.25+4～5％蔗糖。
Surface contamination is the main reason for the high pollution of woody tissue culture. Choosing sunny or cloudy weathering test material or adopting shoot tip culture is an effective measure to reduce the contamination rate and successfully establish aseptic primary culture. Suitable conditions for the first culture of yellow stems were: WPM + BA1.0 + KT0.5 + NAA0.5 + GA_30.25 +4 ~ 5% sucrose.